Xenotransplantation of adult hippocampal neural progenitors into the developing zebrafish for assessment of stem cell plasticity.

Autor: Sandquist EJ; Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, United States of America., Essner JJ; Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, United States of America., Sakaguchi DS; Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, United States of America.; Neuroscience Program, Iowa State University, Ames, Iowa, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2018 May 24; Vol. 13 (5), pp. e0198025. Date of Electronic Publication: 2018 May 24 (Print Publication: 2018).
DOI: 10.1371/journal.pone.0198025
Abstrakt: Adult stem cells are considered multipotent, restricted to differentiate into a few tissue-specific cell types. With the advent of technologies which can dedifferentiate and transdifferentiate cell types, assumptions about the process of cell fate determination must be reconsidered, including the role of extrinsic versus intrinsic factors. To determine the plasticity of adult neural progenitors, rat hippocampal progenitor cells were xenotransplanted into embryonic zebrafish. These animals allow for easy detection of transplanted cells due to their external development and transparency at early stages. Adult neural progenitors were observed throughout the zebrafish for the duration of the experiment (at least five days post-transplantation). While the majority of transplanted cells were observed in the central nervous system, a large percentage of cells were located in superficial tissues. However, approximately one-third of these cells retained neural morphology and expression of the neuronal marker, Class III β-tubulin, indicating that the transplanted adult neural progenitors did not adapt alternate fates. A very small subset of cells demonstrated unique, non-neural flattened morphology, suggesting that adult neural progenitors may exhibit plasticity in this model, though at a very low rate. These findings demonstrate that the developing zebrafish may be an efficient model to explore plasticity of a variety of adult stem cell types and the role of external factors on cell fate.
Competing Interests: JJE owns shares in Recombinetics Inc. and Immusoft Inc. This does not alter our adherence to PLOS ONE policies on sharing data and materials. The revision has been developed in collaboration with all the coauthors, and each author has given approval to the final form of this revision.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje