Revalidation and genetic characterization of new members of Group C (Orthobunyavirus genus, Peribunyaviridae family) isolated in the Americas.

Autor: Nunes MRT; Center for Technological Innovation, Evandro Chagas Institute, Ministry of Health, Ananindeua, Pará, Brazil.; Department of Pathology, University of Texas Medical Branch, Galveston, Texas, United States of America., de Souza WM; Virology Research Center, School of Medicine of Ribeirão Preto of University of São Paulo, Ribeirão Preto, São Paulo, Brazil., Acrani GO; Universidade Federal da Fronteira Sul, Campus Passo Fundo, Rio Grande do Sul, Brazil., Cardoso JF; Center for Technological Innovation, Evandro Chagas Institute, Ministry of Health, Ananindeua, Pará, Brazil., da Silva SP; Center for Technological Innovation, Evandro Chagas Institute, Ministry of Health, Ananindeua, Pará, Brazil., Badra SJ; Virology Research Center, School of Medicine of Ribeirão Preto of University of São Paulo, Ribeirão Preto, São Paulo, Brazil., Figueiredo LTM; Virology Research Center, School of Medicine of Ribeirão Preto of University of São Paulo, Ribeirão Preto, São Paulo, Brazil., Vasconcelos PFDC; Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ministry of Health, Ananindeua, Pará, Brazil.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2018 May 24; Vol. 13 (5), pp. e0197294. Date of Electronic Publication: 2018 May 24 (Print Publication: 2018).
DOI: 10.1371/journal.pone.0197294
Abstrakt: Group C serogroup includes members of the Orthobunyavirus genus (family Peribunyaviridae) and comprises 15 arboviruses that can be associated with febrile illness in humans. Although previous studies described the genome characterization of Group C orthobunyavirus, there is a gap in genomic information about the other viruses in this group. Therefore, in this study, complete genomes of members of Group C serogroup were sequenced or re-sequenced and used for genetic characterization, as well as to understand their phylogenetic and evolutionary aspects. Thus, our study reported the genomes of three new members in Group C virus (Apeu strain BeAn848, Itaqui strain BeAn12797 and Nepuyo strain BeAn10709), as well as re-sequencing of original strains of five members: Caraparu (strain BeAn3994), Madrid (strain BT4075), Murucutu (strain BeAn974), Oriboca (strain BeAn17), and Marituba (strain BeAn15). These viruses presented a typical genomic organization related to members of the Orthobunyavirus genus. Interestingly, all viruses of this serogroup showed an open reading frame (ORF) that encodes the putative nonstructural NSs protein that precedes the nucleoprotein ORF, an unprecedented fact in Group C virus. Also, we confirmed the presence of natural reassortment events. This study expands the genomic information of Group C viruses, as well as revalidates the genomic organization of viruses that were previously reported.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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