| Autor: |
Dörr JA; Laboratório de Cultura de Células, Programa de Pós-graduação em Biotecnologia, Universidade do Vale do Taquari (Univates) , Lajeado , Brazil., Bitencourt S; Laboratório de Cultura de Células, Programa de Pós-graduação em Biotecnologia, Universidade do Vale do Taquari (Univates) , Lajeado , Brazil., Bortoluzzi L; Laboratório de Cultura de Células, Programa de Pós-graduação em Biotecnologia, Universidade do Vale do Taquari (Univates) , Lajeado , Brazil., Alves C; MARE - Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria , Peniche , Portugal., Silva J; MARE - Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria , Peniche , Portugal., Stoll S; Laboratório de Cultura de Células, Programa de Pós-graduação em Biotecnologia, Universidade do Vale do Taquari (Univates) , Lajeado , Brazil., Pinteus S; MARE - Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria , Peniche , Portugal., Boligon AA; Microbiology and Parasitology Department, Federal University of Santa Maria , Santa Maria , Brazil., Santos RCV; Microbiology and Parasitology Department, Federal University of Santa Maria , Santa Maria , Brazil., Laufer S; Department of Pharmaceutical and Medicinal Chemistry, Institute of Pharmacy, University of Tübingen , Tübingen , Germany., Pedrosa R; MARE - Marine and Environmental Sciences Centre, ESTM, Instituto Politécnico de Leiria , Peniche , Portugal., Goettert MI; Laboratório de Cultura de Células, Programa de Pós-graduação em Biotecnologia, Universidade do Vale do Taquari (Univates) , Lajeado , Brazil. |
| Abstrakt: |
Several species of the genus Ceiba (Malvaceae) are ethnopharmacologically used. Thus, this study aimed to investigate the in vitro beneficial properties of the aqueous stem bark extract of Ceiba speciosa . The extract presented a great amount of phenolic compounds (117.4 ± 6.2 mg GAE/g). The antioxidant activity was assessed by DPPH (IC 50 = 42.87 μg/mL), ORAC (2351.17 μmol TE/g) and FRAP (235.94 μM FeSO 4 /g) methods. In addition, the extract reduced MCF-7 cell viability as assessed by MTT. However, it prevented mitochondrial membrane depolarization and reduced caspase-9 activity induced by hydrogen peroxide. In conclusion, these findings indicate the extract is an excellent source of natural antioxidants and is able to protect ROS-induced cell death. Therefore, C. speciosa extract may possess beneficial properties for application in pharmaceutical industry as an antioxidant. However, further studies to better elucidate its mechanisms and to isolate its active compounds are required. |
