The intracellular nucleotide-binding leucine-rich repeat receptor (SlNRC4a) enhances immune signalling elicited by extracellular perception.

Autor: Leibman-Markus M; School of Plant Sciences and Food Security, Tel Aviv University, Tel Aviv, Israel., Pizarro L; School of Plant Sciences and Food Security, Tel Aviv University, Tel Aviv, Israel., Schuster S; School of Plant Sciences and Food Security, Tel Aviv University, Tel Aviv, Israel., Lin ZJD; Department of Plant Pathology, University of California, Davis, California., Gershony O; Department of Plant Pathology and Weed Research ARO, The Volcani Center, Rishon LeZion, Israel., Bar M; Department of Plant Pathology and Weed Research ARO, The Volcani Center, Rishon LeZion, Israel., Coaker G; Department of Plant Pathology, University of California, Davis, California., Avni A; School of Plant Sciences and Food Security, Tel Aviv University, Tel Aviv, Israel.
Jazyk: angličtina
Zdroj: Plant, cell & environment [Plant Cell Environ] 2018 Oct; Vol. 41 (10), pp. 2313-2327. Date of Electronic Publication: 2018 Jul 03.
DOI: 10.1111/pce.13347
Abstrakt: Plant recognition and defence against pathogens employs a two-tiered perception system. Surface-localized pattern recognition receptors (PRRs) act to recognize microbial features, whereas intracellular nucleotide-binding leucine-rich repeat receptors (NLRs) directly or indirectly recognize pathogen effectors inside host cells. Employing the tomato PRR LeEIX2/EIX model system, we explored the molecular mechanism of signalling pathways. We identified an NLR that can associate with LeEIX2, termed SlNRC4a (NB-LRR required for hypersensitive response-associated cell death-4). Co-immunoprecipitation demonstrates that SlNRC4a is able to associate with different PRRs. Physiological assays with specific elicitors revealed that SlNRC4a generally alters PRR-mediated responses. SlNRC4a overexpression enhances defence responses, whereas silencing SlNRC4 reduces plant immunity. Moreover, the coiled-coil domain of SlNRC4a is able to associate with LeEIX2 and is sufficient to enhance responses upon EIX perception. On the basis of these findings, we propose that SlNRC4a acts as a noncanonical positive regulator of immunity mediated by diverse PRRs. Thus, SlNRC4a could link both intracellular and extracellular immune perceptions.
(© 2018 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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