| Autor: |
Bratcher HB; University of Oxford, Department of Zoology, Oxford, United Kingdom., Brehony C; University of Oxford, Department of Zoology, Oxford, United Kingdom.; Clinical Science Institute, National University of Ireland, Galway, Republic of Ireland., Heuberger S; Austrian Agency for Health and Food Safety, Graz, Austria., Pieridou-Bagatzouni D; Nicosia General Hospital, Nicosia, Cyprus., Křížová P; National Institute of Public Health, Prague, Czech Republic., Hoffmann S; Statens Serum Institute, Copenhagen, Denmark., Toropainen M; National Institute for Health and Welfare, Helsinki, Finland., Taha MK; Pasteur Institute, Paris, France., Claus H; University of Würzburg, Würzburg, Germany., Tzanakaki G; National Meningitis Reference Laboratory, Athens, Greece., Erdôsi T; National Center for Epidemiology, Budapest, Hungary., Galajeva J; Riga East University Hospital, Riga, Latvia., van der Ende A; Academic Medical Center, University of Amsterdam, The Netherlands., Skoczyńska A; National Medicines Institute, Warsaw, Poland., Pana M; National Institute of Research and Development for Microbiology and Immunology, Bucharest, Romania., Vaculíková A; Public Health Authority of the Slovak Republic, Bratislava, Slovakia., Paragi M; National Institute of Public Health, Ljubljana, Slovenia., Maiden MC; University of Oxford, Department of Zoology, Oxford, United Kingdom., Caugant DA; Norwegian Institute of Public Health, Oslo, Norway. |
| Abstrakt: |
Invasive meningococcal disease surveillance in Europe combines isolate characterisation and epidemiological data to support public health intervention. A representative European Meningococcal Strain Collection (EMSC) of IMD isolates was obtained, and whole genome sequenced to characterise 799 EMSC isolates from the epidemiological year July 2011-June 2012. To establish a genome library (GL), the isolate information was deposited in the pubMLST.org/neisseria database. Genomes were curated and annotated at 2,429 meningococcal loci, including those defining clonal complex, capsule, antigens, and antimicrobial resistance. Most genomes contained genes encoding B (n = 525; 65.7%) or C (n = 163; 20.4%) capsules; isolates were genetically highly diverse, with >20 genomic lineages, five of which comprising 60.7% (n = 485) of isolates. There were >350 antigenic fine-types: 307 were present once, the most frequent (P1.7-2,4:F5-1) comprised 8% (n = 64) of isolates. Each genome was characterised for Bexsero Antigen Sequence Typing (BAST): 25.5% (n = 204) of isolates contained alleles encoding the fHbp and/or the PorA VR1 vaccine component, but most genomes (n = 513; 64.2%) did not contain the NadA component. EMSC-GL will support an integrated surveillance of disease-associated genotypes in Europe, enabling the monitoring of hyperinvasive lineages, outbreak identification, and supporting vaccine programme implementation. |
