Full-length and defective enterovirus G genomes with distinct torovirus protease insertions are highly prevalent on a Chinese pig farm.

Autor: Wang Y; School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, People's Republic of China., Zhang W; School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, People's Republic of China., Liu Z; School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, People's Republic of China., Fu X; School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, People's Republic of China., Yuan J; School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, People's Republic of China., Zhao J; School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, People's Republic of China., Lin Y; School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, 750000, Ningxia, People's Republic of China., Shen Q; School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, People's Republic of China., Wang X; School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, People's Republic of China., Deng X; Blood Systems Research Institute, San Francisco, California, USA., Delwart E; Blood Systems Research Institute, San Francisco, California, USA., Shan T; Department of Swine Infectious Disease, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, People's Republic of China. shantongling@yahoo.com., Yang S; School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, People's Republic of China. johnsonyang1979@163.com.; School of Medicine, Jiangsu University, 301 Xuefu Road, Zhenjiang, 200240, Jiangsu, People's Republic of China. johnsonyang1979@163.com.
Jazyk: angličtina
Zdroj: Archives of virology [Arch Virol] 2018 Sep; Vol. 163 (9), pp. 2471-2476. Date of Electronic Publication: 2018 May 21.
DOI: 10.1007/s00705-018-3875-x
Abstrakt: Recombination occurs frequently between enteroviruses (EVs) which are classified within the same species of the Picornaviridae family. Here, using viral metagenomics, the genomes of two recombinant EV-Gs (strains EVG 01/NC_CHI/2014 and EVG 02/NC_CHI/2014) found in the feces of pigs from a swine farm in China are described. The two strains are characterized by distinct insertion of a papain-like protease gene from toroviruses classified within the Coronaviridae family. According to recent reports the site of the torovirus protease insertion was located at the 2C/3A junction region in EVG 02/NC_CHI/2014. For the other variant EVG 01/NC_CHI/2014, the inserted protease sequence replaced the entire viral capsid protein region up to the VP1/2A junction. These two EV-G strains were highly prevalent in the same pig farm with all animals shedding the full-length genome (EVG 02/NC_CHI/2014) while 65% also shed the capsid deletion mutant (EVG 01/NC_CHI/2014). A helper-defective virus relationship between the two co-circulating EV-G recombinants is hypothesized.
Databáze: MEDLINE
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