Comparison of Two Genotyping Methods for Distinguishing Recrudescence from Reinfection in Antimalarial Drug Efficacy/Effectiveness Trials.
| Autor: | Fulakeza JRM; University of Malawi, College of Medicine, Blantyre, Malawi., Banda RL; Malawi-Liverpool-Wellcome Trust Clinical Research Programme, Blantyre, Malawi., Lipenga TR; Malawi-Liverpool-Wellcome Trust Clinical Research Programme, Blantyre, Malawi., Terlouw DJ; Liverpool School of Tropical Medicine, Liverpool, United Kingdom.; Malawi-Liverpool-Wellcome Trust Clinical Research Programme, Blantyre, Malawi., Nkhoma SC; Malawi-Liverpool-Wellcome Trust Clinical Research Programme, Blantyre, Malawi.; Liverpool School of Tropical Medicine, Liverpool, United Kingdom., Hodel EM; Liverpool School of Tropical Medicine, Liverpool, United Kingdom.; Malawi-Liverpool-Wellcome Trust Clinical Research Programme, Blantyre, Malawi. |
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| Jazyk: | angličtina |
| Zdroj: | The American journal of tropical medicine and hygiene [Am J Trop Med Hyg] 2018 Jul; Vol. 99 (1), pp. 84-86. Date of Electronic Publication: 2018 May 17. |
| DOI: | 10.4269/ajtmh.18-0002 |
| Abstrakt: | Genotyping of allelic variants of Plasmodium falciparum merozoite surface proteins 1 and 2 ( msp-1 and msp-2 ), and the glutamate-rich protein is the gold standard for distinguishing reinfections from recrudescences in antimalarial drug trials. We compared performance of the recently developed 24-single-nucleotide polymorphism (SNP) Barcoding Assay against msp-1 and msp-2 genotyping in a cluster-randomized effectiveness trial of artemether-lumefantrine and dihydroartemisinin-piperaquine in Malawi. Rates of recrudescence and reinfection estimated by the two methods did not differ significantly (Fisher's exact test; P = 0.887 and P = 0.768, respectively). There was a strong agreement between the two methods in predicting treatment outcomes and resolving the genetic complexity of malaria infections in this setting. These results support the use of this SNP assay as an alternative method for correcting antimalarial efficacy/effectiveness data. |
| Databáze: | MEDLINE |
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