Insights from the Molecular Modelling and Docking Analysis of AIF-NLS complex to infer Nuclear Translocation of the Protein.

Autor:	Srivaths A; Department of Genetic Engineering, School of Bioengineering, SRM University, Kattankulathur, Chennai - 603203., Ramanathan S; Department of Genetic Engineering, School of Bioengineering, SRM University, Kattankulathur, Chennai - 603203., Sakthivel S; Department of Genetic Engineering, School of Bioengineering, SRM University, Kattankulathur, Chennai - 603203., Habeeb S; Department of Genetic Engineering, School of Bioengineering, SRM University, Kattankulathur, Chennai - 603203.
Jazyk:	angličtina
Zdroj:	Bioinformation [Bioinformation] 2018 Mar 31; Vol. 14 (3), pp. 132-139. Date of Electronic Publication: 2018 Mar 31 (Print Publication: 2018).
DOI:	10.6026/97320630014132
Abstrakt:	Apoptosis Inducing Factor protein has a dual role depending on its localization in mitochondrion (energy production) and nucleus (induces apoptosis). Cell damage transports this protein to nucleus which otherwise favors mitochondrion. The alteration of Nuclear Localisation Signal tags could aid nuclear translocation. In this study, apoptosis inducing factor protein (AIF) was conjugated with strong NLS tags and its binding affinity with Importin was studied using in silico approaches such as molecular modeling and docking. This aims to improve the docking affinity of the AIF-Importin complex thus allowing for nuclear translocation, in order to induce caspase-independent apoptosis of the cell.
Databáze:	MEDLINE
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