Graphene oxide enhances alginate encapsulated cells viability and functionality while not affecting the foreign body response.

Autor: Ciriza J; a Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine , CIBER-BBN , Vitoria-Gasteiz , Spain.; b NanoBioCel Group, Laboratory of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy , University of the Basque Country, UPV/EHU , Vitoria-Gasteiz , Spain., Saenz Del Burgo L; a Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine , CIBER-BBN , Vitoria-Gasteiz , Spain.; b NanoBioCel Group, Laboratory of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy , University of the Basque Country, UPV/EHU , Vitoria-Gasteiz , Spain., Gurruchaga H; a Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine , CIBER-BBN , Vitoria-Gasteiz , Spain.; b NanoBioCel Group, Laboratory of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy , University of the Basque Country, UPV/EHU , Vitoria-Gasteiz , Spain., Borras FE; c REMAR-IVECAT Group, Health Science Research Institute Germans Trias i Pujol , Badalona , Spain.; d Department of Cell Biology, Physiology and Immunology , Universitat Autònoma de Barcelona , Bellaterra , Spain.; e Nephrology Service, Germans Trias i Pujol University Hospital , Badalona , Spain., Franquesa M; c REMAR-IVECAT Group, Health Science Research Institute Germans Trias i Pujol , Badalona , Spain.; e Nephrology Service, Germans Trias i Pujol University Hospital , Badalona , Spain., Orive G; a Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine , CIBER-BBN , Vitoria-Gasteiz , Spain.; b NanoBioCel Group, Laboratory of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy , University of the Basque Country, UPV/EHU , Vitoria-Gasteiz , Spain., Hernández RM; a Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine , CIBER-BBN , Vitoria-Gasteiz , Spain.; b NanoBioCel Group, Laboratory of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy , University of the Basque Country, UPV/EHU , Vitoria-Gasteiz , Spain., Pedraz JL; a Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine , CIBER-BBN , Vitoria-Gasteiz , Spain.; b NanoBioCel Group, Laboratory of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy , University of the Basque Country, UPV/EHU , Vitoria-Gasteiz , Spain.
Jazyk: angličtina
Zdroj: Drug delivery [Drug Deliv] 2018 Nov; Vol. 25 (1), pp. 1147-1160.
DOI: 10.1080/10717544.2018.1474966
Abstrakt: The combination of protein-coated graphene oxide (GO) and microencapsulation technology has moved a step forward in the challenge of improving long-term alginate encapsulated cell survival and sustainable therapeutic protein release, bringing closer its translation from bench to the clinic. Although this new approach in cell microencapsulation represents a great promise for long-term drug delivery, previous studies have been performed only with encapsulated murine C 2 C 12 myoblasts genetically engineered to secrete murine erythropoietin (C 2 C 12 -EPO) within 160 µm diameter hybrid alginate protein-coated GO microcapsules implanted into syngeneic mice. Here, we show that encapsulated C 2 C 12 -EPO myoblasts survive longer and release more therapeutic protein by doubling the micron diameter of hybrid alginate-protein-coated GO microcapsules to 380 µm range. Encapsulated mesenchymal stem cells (MSC) genetically modified to secrete erythropoietin (D1-MSCs-EPO) within 380 µm-diameter hybrid alginate-protein-coated GO microcapsules confirmed this improvement in survival and sustained protein release in vitro. This improved behavior is reflected in the hematocrit increase of allogeneic mice implanted with both encapsulated cell types within 380 µm diameter hybrid alginate-protein-coated GO microcapsules, showing lower immune response with encapsulated MSCs. These results provide a new relevant step for the future clinical application of protein-coated GO on cell microencapsulation.
Databáze: MEDLINE
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