Expression, Purification and Characterization of a GII.4 Norovirus Protease from Minerva Virus.

Autor:	Kuiper BD; Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, MI 48201, United States., Muzzarelli KM; Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, MI 48201, United States., Keusch BJ; Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, MI 48201, United States., Holcomb J; Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, MI 48201, United States., Amblard F; Center for AIDS Research, Laboratory of Biochemical Pharmacology, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, United States., Liu P; Center for AIDS Research, Laboratory of Biochemical Pharmacology, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, United States., Zhou S; Center for AIDS Research, Laboratory of Biochemical Pharmacology, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, United States., Kovari IA; Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, MI 48201, United States., Yang Z; Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, MI 48201, United States., Schinazi RF; Center for AIDS Research, Laboratory of Biochemical Pharmacology, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, United States., Kovari LC; Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, Detroit, MI 48201, United States.
Jazyk:	angličtina
Zdroj:	Infectious disorders drug targets [Infect Disord Drug Targets] 2018; Vol. 18 (3), pp. 224-232.
DOI:	10.2174/1871526518666180521091158
Abstrakt:	Background: Noroviruses are the leading cause of acute gastroenteritis worldwide. Norovirus proteases, which are responsible for cleavage of the viral polyprotein, have become an attractive drug target to treat norovirus infections. Genogroup II (GII) noroviruses are responsible for a majority of outbreaks; however, limited data exists regarding GII norovirus proteases. Methods: We report here successful expression, purification, characterization, and inhibition of the Minerva virus protease (MVpro), a genogroup II genotype 4 (GII.4) norovirus protease. We observed MVpro as both a monomer and dimer in solution through sizeexclusion chromatography. In addition, MVpro cleaves the synthetic substrate mimicking the MVpro NS2/NS3 cleavage site more efficiently than other norovirus proteases such as the Norwalk virus protease (GI.1) and the MD145 protease (GII.4). Results and Conclusion: Compound A, a potent inhibitor of MVpro, is a good starting point for the design of inhibitors to target GII.4 noroviruses. Furthermore, the results presented here will allow for future characterization of MVpro inhibitors as they are synthesized. (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu