Safety assessment of Morus nigra L. leaves: Acute and subacute oral toxicity studies in Wistar rats.

Autor: Figueredo KC; Department of Physiology and Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil. Electronic address: kassia.tquimica@yahoo.com.br., Guex CG; Department of Physiology and Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil., Reginato FZ; Department of Physiology and Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil., Haas da Silva AR; Department of Physiology and Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil., Cassanego GB; Department of Physiology and Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil., Lhamas CL; Veterinary Hospital, Federal University of Santa Maria, Santa Maria, RS, Brazil., Boligon AA; Department of Physiology and Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil., Lopes GHH; Department of Food Science and Technology, Federal University of Santa Maria, Santa Maria, RS, Brazil., de Freitas Bauermann L; Department of Physiology and Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2018 Oct 05; Vol. 224, pp. 290-296. Date of Electronic Publication: 2018 May 14.
DOI: 10.1016/j.jep.2018.05.013
Abstrakt: Ethnopharmacological Relevance: Morus nigra L. is a plant native to Asia, and well adapted to the Brazilian climate. It is popularly known as "amoreira preta", and is part of the National List of Plants of Interest to the Brazilian Unified Health System. It is used in folk medicine mainly to soften the effects of menopause, as anti-inflammatory, antidiabetic and antihypertensive. However, information on safe doses and use is still precarious.
Aim of the Study: To identify the chemical composition of the ethanolic extract of Morus nigra L. leaves (EEMN), as well as perform a toxicological study in male and female rats.
Materials and Methods: The chemical composition of the extract was performed by HPLC/DAD. In the acute study, the dose administered was 2000 mg/kg, and signs of toxicity and mortality was observed. In the sub-acute study, the extract was administered at doses of 500, 750 and 1000 mg/kg for 28 days. Behavioral changes, object recognition test, renal and hepatic tissue assessments, biochemical and hematological parameters were determined. The extract was administered orally to male and female rats in both studies.
Results: Quercetin and caffeic acid showed as major compounds in the extract. In the acute treatment, the extract was classified as safe (category 5), according to the protocol. In the subacute study, there was a decrease in AST in males (750 and 1000 mg/kg) and females (1000 mg/kg), reduction of total cholesterol in females (750 and 1000 mg/kg), and increase in renal and hepatic change the LPO levels.
Conclusion: The present investigation showed that EEMN did not present significant toxic effects when administered orally. Moreover, presented a potentially protective action of organs and possesses hypocholesterolemic activity, thus, it is shown as a promising natural source to be used in pharmacology.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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