Association of the placental VEGF promoter polymorphisms and VEGF mRNA expression with preeclampsia.

Autor: Keshavarzi F; a Department of Clinical Biochemistry, School of Medicine , Zahedan University of Medical Sciences , zahedan , Iran (the Islamic Republic of)., Shahrakipoor M; b Department of Biostatistics and Epidemiology, School of Public Health , Zahedan University of Medical Sciences , zahedan , Iran (the Islamic Republic of)., Teimoori B; c Department of Obstetrics and Gynecology, School of Medicine , Zahedan University of Medical Sciences , Zahedan , Iran (the Islamic Republic of)., Yaghmaei M; d Department of Obstetrics and Gynecology, School of Medicine , Shahid Beheshty University of Medical Sciences , tehran , Iran (the Islamic Republic of)., Narooei-Nejad M; e Department of Genetics, School of Medicine , Zahedan University of Medical Sciences , Zahedan , Iran (the Islamic Republic of)., Rasooli A; f Department of Laboratory Science, Paramedical School , zahedan , Iran (the Islamic Republic of)., Salimi S; a Department of Clinical Biochemistry, School of Medicine , Zahedan University of Medical Sciences , zahedan , Iran (the Islamic Republic of).; g Cellular and Molecular Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences , Zahedan , Iran (the Islamic Republic of).
Jazyk: angličtina
Zdroj: Clinical and experimental hypertension (New York, N.Y. : 1993) [Clin Exp Hypertens] 2019; Vol. 41 (3), pp. 274-279. Date of Electronic Publication: 2018 May 17.
DOI: 10.1080/10641963.2018.1469644
Abstrakt: Background: Preeclampsia (PE), is a pregnancy-specific complication with the placental origin which associated with altered expression of angiogenic factors. Vascular Endothelial Growth Factor (VEGF), is a growth and hypoxia-induced factor which contributes to the regulation of various processes. The present study has investigated the association of the placental VEGF -634G/C (rs2010963), -1154G/A (rs1570360), and -2549 I/D (18bpindel) polymorphisms in the promoter region with VEGF mRNA expression in PE women and control group.
Methods: This case-control study was performed on the placenta of 84 PE women and 103 controls after delivery. Genotyping of the VEGF polymorphisms was done by PCR or PCR, PCR-RFLP or sequencing methods. The mRNA expression levels were measured by Quantitative Real-Time PCR.
Results: The relative mRNA expression of VEGF gene was significantly higher in PE women compared to controls. The relative mRNA expression of VEGF gene was significantly higher in women with -634CC genotype compared to CG + GG genotypes in PE women and total studied women but not in control women. However, there was no association between the placental VEGF -1154G/A and -2549 I/D polymorphisms and VEGF mRNA expression neither in PE nor in control groups.
Conclusion: The current study found higher mRNA expression of placental VEGF gene in PE women. The mRNA expression of the placental VEGF gene has been up-regulated in the placenta of women with -634CC genotype. No association was found between the placental VEGF -1154G/A and -2549 I/D polymorphisms and VEGF mRNA expression.
Databáze: MEDLINE
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