| Autor: |
Chen Y; College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,People's Republic of China., Zhang H; College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,People's Republic of China., Cheng Y; College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,People's Republic of China., Li Y; College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,People's Republic of China., Wen C; College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,People's Republic of China., Zhou Y; College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,People's Republic of China. |
| Jazyk: |
angličtina |
| Zdroj: |
The British journal of nutrition [Br J Nutr] 2018 Jun; Vol. 119 (11), pp. 1254-1262. |
| DOI: |
10.1017/S0007114518000740 |
| Abstrakt: |
This study was conducted to investigate the protective effects of l-threonine (l-Thr) supplementation on growth performance, inflammatory responses and intestinal barrier function of young broilers challenged with lipopolysaccharide (LPS). A total of 144 1-d-old male chicks were allocated to one of three treatments: non-challenged broilers fed a basal diet (control group), LPS-challenged broilers fed a basal diet without l-Thr supplementation and LPS-challenged broilers fed a basal diet supplemented with 3·0 g/kg l-Thr. LPS challenge was performed intraperitoneally at 17, 19 and 21 d of age, whereas the control group received physiological saline injection. Compared with the control group, LPS challenge impaired growth performance of broilers, and l-Thr administration reversed LPS-induced increase in feed/gain ratio. LPS challenge elevated blood cell counts related to inflammation, and pro-inflammatory cytokine concentrations in serum (IL-1β and TNF-α), spleen (IL-1β and TNF-α) and intestinal mucosa (jejunal interferon-γ (IFN-γ) and ileal IL-1β). The concentrations of intestinal cytokines in LPS-challenged broilers were reduced by l-Thr supplementation. LPS administration increased circulating d-lactic acid concentration, whereas it reduced villus height, the ratio between villus height and crypt depth and goblet density in both jejunum and ileum. LPS-induced decreases in jejunal villus height, intestinal villus height:crypt depth ratio and ileal goblet cell density were reversed with l-Thr supplementation. Similarly, LPS-induced alterations in the intestinal mRNA abundances of genes related to intestinal inflammation and barrier function (jejunal toll-like receptor 4, IFN- γ and claudin-3, and ileal IL-1 β and zonula occludens-1) were normalised with l-Thr administration. It can be concluded that l-Thr supplementation could attenuate LPS-induced inflammatory responses and intestinal barrier damage of young broilers. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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