Does lipid peroxidation and oxidative DNA damage differ in cryopreserved semen samples from young, adult and aged Nellore bulls?

Autor: Trevizan JT; Department of Medicine, Surgery and Animal Reproduction, School of Veterinary Medicine, São Paulo State University - UNESP, Araçatuba, São Paulo, 16050-680, Brazil. Electronic address: juliane.t.teramachi@hotmail.com., Carreira JT; Department of Medicine, Surgery and Animal Reproduction, School of Veterinary Medicine, São Paulo State University - UNESP, Araçatuba, São Paulo, 16050-680, Brazil. Electronic address: janababel@hotmail.com., Carvalho IR; Department of Medicine, Surgery and Animal Reproduction, School of Veterinary Medicine, São Paulo State University - UNESP, Araçatuba, São Paulo, 16050-680, Brazil. Electronic address: isadoraresende@hotmail.com., Kipper BH; Department of Medicine, Surgery and Animal Reproduction, School of Veterinary Medicine, São Paulo State University - UNESP, Araçatuba, São Paulo, 16050-680, Brazil. Electronic address: brunakipper@hotmail.com., Nagata WB; Department of Support, Production and Animal Health, School of Veterinary Medicine, São Paulo State University - UNESP, Araçatuba, São Paulo, 16050-680, Brazil. Electronic address: walter.bn@hotmail.com., Perri SHV; Department of Support, Production and Animal Health, School of Veterinary Medicine, São Paulo State University - UNESP, Araçatuba, São Paulo, 16050-680, Brazil. Electronic address: shvperri@fmva.unesp.br., Franco Oliveira ME; Department of Preventative Veterinary Medicine and Animal Reproduction, School of Agricultural and Veterinary Sciences, São Paulo State University - UNESP, Jaboticabal, São Paulo, 14884-900, Brazil. Electronic address: m_emiliafraoli@yahoo.com.br., Pierucci JC; Department of Preventative Veterinary Medicine and Animal Reproduction, School of Agricultural and Veterinary Sciences, São Paulo State University - UNESP, Jaboticabal, São Paulo, 14884-900, Brazil. Electronic address: juliapierucci@hotmail.com., Koivisto MB; Department of Medicine, Surgery and Animal Reproduction, School of Veterinary Medicine, São Paulo State University - UNESP, Araçatuba, São Paulo, 16050-680, Brazil. Electronic address: koivisto@fmva.unesp.br.
Jazyk: angličtina
Zdroj: Animal reproduction science [Anim Reprod Sci] 2018 Aug; Vol. 195, pp. 8-15. Date of Electronic Publication: 2018 Apr 17.
DOI: 10.1016/j.anireprosci.2018.04.071
Abstrakt: The aims of this study were to evaluate cryopreserved semen of Nellore bulls of different ages and verify whether sperm quality declines with advancing age and whether lipid peroxidation and DNA damage are involved in this process. For this purpose, 40 Nellore bulls were divided into three age groups: Young, aged 1.8-2 years (n = 9); Adult, aged 3.5-7.0 years (n = 19); and Seniors, aged 8.0-14.3 years (n = 12). Three ejaculates were collected from each bull, cryopreserved and evaluated for various parameters including membrane integrity, mitochondrial potential (FITC-PSA and JC1), lipid peroxidation (C-11BODIPY 581 / 591) and oxidative DNA damage (8OHdG) using flow cytometry. The thawed semen of senior bulls was characterized by a low percentage of motile sperm (33.7 ± 6.1%), higher damage to the plasma and acrosomal membrane (37.5 ± 9.8%), and low mitochondrial potential (29.1 ± 13.8%), as well as higher percentages of peroxidated cells (53.6 ± 12.2%) and DNA damage (44.1 ± 11.0%; P < 0.05). Lipid peroxidation was negatively correlated with motility (r = -0.35, P < 0.0002), average mitochondrial potential (r = -0.42; P < 0.0001) and showed a positive correlation with membrane injury and oxidative DNA damage (r = 039; P = 0.0003). Young bulls presented superior thawed sperm quality, possibly due to greater resistance to oxidative stress and, consequently, to cryopreservation. In conclusion, the sperm quality of bull semen declines with advancing age and is strongly associated with increased oxidative damage to both the plasma membrane and DNA.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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