Development of an in-house mixed-mode solid-phase extraction for the determination of 16 basic drugs in urine by High Performance Liquid Chromatography-Ion Trap Mass Spectrometry.

Autor: Musile G; Department of Diagnostics and Public Health, Section of Legal Medicine, University of Verona, Verona, Italy., Cenci L; Department of Biotechnology, University of Verona, Verona, Italy., Piletska E; Chemistry Department, University of Leicester, Leicester, UK., Gottardo R; Department of Diagnostics and Public Health, Section of Legal Medicine, University of Verona, Verona, Italy., Bossi AM; Department of Biotechnology, University of Verona, Verona, Italy., Bortolotti F; Department of Diagnostics and Public Health, Section of Legal Medicine, University of Verona, Verona, Italy. Electronic address: federica.bortolotti@univr.it.
Jazyk: angličtina
Zdroj: Journal of chromatography. A [J Chromatogr A] 2018 Jul 27; Vol. 1560, pp. 10-18. Date of Electronic Publication: 2018 May 09.
DOI: 10.1016/j.chroma.2018.05.019
Abstrakt: The aim of the present work was to develop a novel in-house mixed-mode SPE sorbent to be used for the HPLC-Ion TrapMS determination of 16 basic drugs in urine. By using a computational modelling, a virtual monomer library was screened identifying three suitable functional monomers, methacrylic acid (MAA), itaconic acid (IA) and 2-acrylamide-2-methylpropane sulfonic acid (AMPSA), respectively. Three different sorbents were then synthetized based on these monomers, and using as cross-linker trimethylolpropane trimethacrylate (TMPTMA). The sorbent characterization analyses brought to the selection of the AMPSA based phase. Using this novel in-house sorbent, a SPE-HPLC-Ion TrapMS method for drug analysis in urine was validated proving to be selective and accurate and showing a sensitivity adequate for toxicological urine analysis. The comparison of the in-house mixed-mode SPE sorbent with two analogous commercial mixed-mode SPE phases showed that the first one was better not only in terms of process efficiency, but also in terms of quality-price rate. To the best of our knowledge, this is the first time in which an in-house SPE procedure has been applied to the toxicological analysis of a complex matrix, such as urine.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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