Influence of nitrogen on growth, biomass composition, production, and properties of polyhydroxyalkanoates (PHAs) by microalgae.

Autor: Costa SS; Institute of Health Sciences, RENORBIO, Federal University of Bahia, Salvador, Bahia, Brazil. Electronic address: manthasc@yahoo.com.br., Miranda AL; Institute of Health Sciences, RENORBIO, Federal University of Bahia, Salvador, Bahia, Brazil., Andrade BB; Institute of Health Sciences, Federal University of Bahia, Salvador, Bahia, Brazil., Assis DJ; Department of Chemical Engineering, Polytechnic School, Federal University of Bahia, Salvador, Bahia, Brazil., Souza CO; Department of Bromatological Analysis, College of Pharmacy, Federal University of Bahia, Salvador, Bahia, Brazil., de Morais MG; Laboratory of Biochemical Engineering, College of Chemistry and Food Engineering, Federal University of Rio Grande, Rio Grande, Brazil., Costa JAV; Laboratory of Biochemical Engineering, College of Chemistry and Food Engineering, Federal University of Rio Grande, Rio Grande, Brazil., Druzian JI; Department of Bromatological Analysis, College of Pharmacy, Federal University of Bahia, Salvador, Bahia, Brazil.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2018 Sep; Vol. 116, pp. 552-562. Date of Electronic Publication: 2018 May 12.
DOI: 10.1016/j.ijbiomac.2018.05.064
Abstrakt: This study sought to evaluate influence of nitrogen availability on cell growth, biomass composition, production, and the properties of polyhydroxyalkanoates during cultivation of microalgae Chlorella minutissima, Synechococcus subsalsus, and Spirulina sp. LEB-18. The cellular growth of microalgae reduced with the use of limited nitrogen medium, demonstrating that nitrogen deficiency interferes with the metabolism of microorganisms and the production of biomass. The biochemical composition of microalgae was also altered, which was most notable in the degradation of proteins and chlorophylls and the accumulation of carbonaceous storage molecules such as lipids and polyhydroxyalkanoates. Chlorella minutissima did not produce these polymers even in a nitrogen deficient environment. The largest accumulations of the polyhydroxyalkanoates occurred after a 15 days culture, with a concentration of 16% (dry cell weight) produced by the Synechococcus subsalsus strain and 12% by Spirulina sp. LEB-18. Polyhydroxyalkanoates produced by Synechococcus subsalsus and Spirulina sp. LEB-18 presented different thermal and physical properties, indicating the influence of producing strain on polyhydroxyalkanoates properties. The polymers obtained consisted of long chain monomers with 14 to 18 carbon atoms. This composition is novel, as it has not previously been found in PHAs obtained from Synechococcus subsalsus and Spirulina sp. LEB-18.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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