Laser Ablation Electrospray Ionization-Mass Spectrometry Imaging (LAESI-MS) for Spatially Resolved Plant Metabolomics.

Autor:	Etalo DW; Netherlands Institute of Ecology, Wageningen, The Netherlands., Díez-Simón C; Laboratory of Plant Physiology, Wageningen University and Research, Wageningen, The Netherlands., de Vos RCH; Business Unit Bioscience, Wageningen University and Research, Wageningen, The Netherlands.; Netherlands Metabolomics Centre, Leiden, The Netherlands., Hall RD; Laboratory of Plant Physiology, Wageningen University and Research, Wageningen, The Netherlands. robert.hall@wur.nl.; Business Unit Bioscience, Wageningen University and Research, Wageningen, The Netherlands. robert.hall@wur.nl.; Netherlands Metabolomics Centre, Leiden, The Netherlands. robert.hall@wur.nl.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1778, pp. 253-267.
DOI:	10.1007/978-1-4939-7819-9_18
Abstrakt:	There is increasing demand to bring the level of metabolomics analyses down to the tissue or cell level. Significant progress has been made involving the use of in situ metabolomics imaging techniques where no tissue collection or extraction is needed prior to analysis. In this chapter we describe a relatively new method which is simple and easy to use. No ectopic matrix or vacuum is required, and analyses are performed with living plant materials directly from (or even still attached to) the plant. Although relatively straightforward, there are still a few caveats as regards this method which are described at the end of the chapter.
Databáze:	MEDLINE
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