Standardization of deep partial-thickness scald burns in C57BL/6 mice.

Autor:	Medina JL; Dental and Craniofacial Trauma Research and Tissue Regeneration Directorate, United States Army Institute of Surgical Research Fort Sam Houston, 78234, Texas, USA., Fourcaudot AB; Dental and Craniofacial Trauma Research and Tissue Regeneration Directorate, United States Army Institute of Surgical Research Fort Sam Houston, 78234, Texas, USA., Sebastian EA; Dental and Craniofacial Trauma Research and Tissue Regeneration Directorate, United States Army Institute of Surgical Research Fort Sam Houston, 78234, Texas, USA., Shankar R; Dental and Craniofacial Trauma Research and Tissue Regeneration Directorate, United States Army Institute of Surgical Research Fort Sam Houston, 78234, Texas, USA., Brown AW; Comparative Pathology Branch, United States Army Institute of Surgical Research Fort Sam Houston, 78234, Texas, USA., Leung KP; Dental and Craniofacial Trauma Research and Tissue Regeneration Directorate, United States Army Institute of Surgical Research Fort Sam Houston, 78234, Texas, USA.
Jazyk:	angličtina
Zdroj:	International journal of burns and trauma [Int J Burns Trauma] 2018 Apr 05; Vol. 8 (2), pp. 26-33. Date of Electronic Publication: 2018 Apr 05 (Print Publication: 2018).
Abstrakt:	Mouse burn models are used to understand the wound healing process and having a reproducible model is important. The different protocols used by researchers can lead to differences in depth of partial-thickness burn wounds. Additionally, standardizing a protocol for mouse burns in the laboratory for one strain may result in substantially different results in other strains. In our current study we describe the model development of a deep partial-thickness burn in C57BL/6 mice using hot water scalding as the source of thermal injury. As part of our model development we designed a template with specifications to allow for even contact of bare mouse skin (2×3 cm) with hot water while protecting the rest of the mouse. Burn depth was evaluated with H&E, Masson's trichrome, and TUNEL staining. Final results were validated with pathology analysis. A water temperature of 54°C with a scalding time of 20 seconds produced consistent deep partial-thickness burns with available equipment described. Other than temperature and time, factors such as template materials and cooling steps after the burn could affect the uniformity of the burns. These findings are useful to burn research by providing some key parameters essential for researchers to simplify the development of their own mouse burn models. Competing Interests: None.
Databáze:	MEDLINE
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