The ascent of man(made oxidoreductases).

Autor: Grayson KJ; School of Biochemistry, Biomedical Sciences Building, University of Bristol, BS8 1TD, UK., Anderson JR; School of Biochemistry, Biomedical Sciences Building, University of Bristol, BS8 1TD, UK; BrisSynBio Synthetic Biology Research Centre, Life Sciences Building, University of Bristol, Tyndall Avenue, Bristol BS8 1TQ, UK. Electronic address: ross.anderson@bristol.ac.uk.
Jazyk: angličtina
Zdroj: Current opinion in structural biology [Curr Opin Struct Biol] 2018 Aug; Vol. 51, pp. 149-155. Date of Electronic Publication: 2018 May 10.
DOI: 10.1016/j.sbi.2018.04.008
Abstrakt: Though established 40 years ago, the field of de novo protein design has recently come of age, with new designs exhibiting an unprecedented level of sophistication in structure and function. With respect to catalysis, de novo enzymes promise to revolutionise the industrial production of useful chemicals and materials, while providing new biomolecules as plug-and-play components in the metabolic pathways of living cells. To this end, there are now de novo metalloenzymes that are assembled in vivo, including the recently reported C45 maquette, which can catalyse a variety of substrate oxidations with efficiencies rivalling those of closely related natural enzymes. Here we explore the successful design of this de novo enzyme, which was designed to minimise the undesirable complexity of natural proteins using a minimalistic bottom-up approach.
(Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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