Keeping it together: absence of genetic variation and DNA incorporation by the predatory bacteria Micavibrio aeruginosavorus and Bdellovibrio bacteriovorus during predation.

Autor: Gupta S; Department of Oral Biology, Rutgers School of Dental Medicine, Newark, NJ 07101, USA., Lemenze A; Department of Medicine and the Center for Emerging Pathogens, Rutgers, New Jersey Medical School, Newark, NJ 07101, USA; Molecular Resource Facility, Rutgers, New Jersey Medical School, Newark, NJ 07101, USA., Donnelly RJ; Molecular Resource Facility, Rutgers, New Jersey Medical School, Newark, NJ 07101, USA., Connell ND; Department of Medicine and the Center for Emerging Pathogens, Rutgers, New Jersey Medical School, Newark, NJ 07101, USA., Kadouri DE; Department of Oral Biology, Rutgers School of Dental Medicine, Newark, NJ 07101, USA. Electronic address: kadourde@sdm.rutgers.edu.
Jazyk: angličtina
Zdroj: Research in microbiology [Res Microbiol] 2018 May - Jun; Vol. 169 (4-5), pp. 237-243. Date of Electronic Publication: 2018 May 08.
DOI: 10.1016/j.resmic.2018.03.002
Abstrakt: The use of predatory bacteria as a potential live therapeutic to control human infection is gaining increased attention. Earlier work with Micavibrio spp. and Bdellovibrio spp. has demonstrated the ability of these predators to control drug-resistant Gram-negative pathogens, Tier-1 select agents and biofilms. Additional studies also confirmed that introducing high doses of the predators into animals does not negatively impact animal well-being and might assist in reducing bacterial burden in vivo. The survival of predators requires extreme proximity to the prey cell, which might bring about horizontal transfer of genetic material, such as genes encoding for pathogenic genetic islands that would indirectly facilitate the spread of genetic material to other organisms. In this study, we examined the genetic makeup of several lab isolates of the predators Bdellovibriobacteriovorus and Micavibrioaeruginosavorus that were cultured repeatedly and stored over a course of 13 years. We also conducted controlled experiments in which the predators were sequentially co-cultured on Klebsiella pneumoniae followed by genetic analysis of the predator. In both cases, we saw little genetic variation and no evidence of horizontally transferred chromosomal DNA from the prey during predator-prey interaction. Culturing the predators repeatedly did not cause any change in predation efficacy.
(Copyright © 2018 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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