| Autor: |
Hu T; Institute of Bionanotechnology and Tissue Engineering, College of Life Sciences, Hunan University, Changsha, 410082, China., Qahtan ASA; Institute of Bionanotechnology and Tissue Engineering, College of Life Sciences, Hunan University, Changsha, 410082, China., Lei L; Department of Orthodontics, Xiangya Stomatological Hospital, Central South University, Changsha, 410008, China., Lei Z; Changsha DMY Medical Technology Co., Ltd, Changsha, 410005, China., Zhao D; Institute of Bionanotechnology and Tissue Engineering, College of Life Sciences, Hunan University, Changsha, 410082, China., Nie H; Institute of Bionanotechnology and Tissue Engineering, College of Life Sciences, Hunan University, Changsha, 410082, China.; Shenzhen Research Institute of Hunan University, Nanshan Hi-new Technology and Industry Park, Shenzhen, 518057, China. |
| Abstrakt: |
In order to improve the release pattern of chemotherapy drug and reduce the possibility of drug resistance, poly(ethylene glycol amine) (PEG)-modified alginate microparticles (ALG-PEG MPs) were developed then two different mechanisms were employed to load doxorubicin (Dox): 1) forming Dox/ALG-PEG complex by electrostatic attractions between unsaturated functional groups in Dox and ALG-PEG; 2) forming Dox-ALG-PEG complex through EDC-reaction between the amino and carboxyl groups in Dox and ALG, respectively. Additionally, tuftsin (TFT), a natural immunomodulation peptide, was conjugated to MPs in order to enhance the efficiency of cellular uptake. It was found that the Dox-ALG-PEG-TFT MPs exhibited a significantly slower release of Dox than Dox/ALG-PEG-TFT MPs in neutral medium, suggesting the role of covalent bonding in prolonging Dox retention. Besides, the release of Dox from these MPs was pH-sensitive, and the release rate was observably increased at pH 6.5 compared to the case at pH 7.4. Compared with Dox/ALG-PEG MPs and Dox-ALG-PEG MPs, their counterparts further conjugated with TFT more efficiently inhibited the growth of HeLa cells over a period of 48 h, implying the effectiveness of TFT in enhancing cellular uptake of MPs. Over a period of 48 h, Dox-ALG-PEG-TFT MPs inhibited the growth of HeLa cells less efficiently than Dox/ALG-PEG-TFT MPs but the difference was not significant ( p > 0.05). In consideration of the prolonged and sustained release of Dox, Dox-ALG-PEG-TFT MPs possess the advantages for long-term treatment. |
