| Autor: |
Sánchez-Sánchez R; SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040, Madrid, Spain., Ferre I; SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040, Madrid, Spain., Re M; Department of Animal Medicine and Surgery, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040, Madrid, Spain., Regidor-Cerrillo J; SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040, Madrid, Spain., Blanco-Murcia J; Department of Animal Medicine and Surgery, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040, Madrid, Spain., Ferrer LM; Departamento de Patología Animal, Facultad de Veterinaria, C/Miguel Servet 177, 50013, Zaragoza, Spain., Navarro T; Departamento de Patología Animal, Facultad de Veterinaria, C/Miguel Servet 177, 50013, Zaragoza, Spain., Pizarro Díaz M; Department of Animal Medicine and Surgery, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040, Madrid, Spain., González-Huecas M; Department of Animal Medicine and Surgery, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040, Madrid, Spain., Tabanera E; Department of Animal Medicine and Surgery, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040, Madrid, Spain., Benavides J; Livestock Health and Production Institute (ULE-CSIC), 24346, León, Spain., Ortega-Mora LM; SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040, Madrid, Spain. luis.ortega@ucm.es. |
| Abstrakt: |
Experimental infections in pregnant sheep have been focused on studying the effect of the time of challenge on the outcome of N. caninum infection, whereas the impact of the dose and route of challenge has not been studied in depth. Therefore, clinical outcome, immune responses, parasite detection and burden, and lesion severity in placental tissues and foetal brains were investigated in 90-day-pregnant sheep inoculated intravenously with 10 5 (G1), 10 4 (G2), 10 3 (G3), or 10 2 (G4) tachyzoites or subcutaneously with 10 4 (G5) tachyzoites of the virulent Nc-Spain7 isolate and an uninfected group (G6). Comparing challenge doses, G1 was the only group that had 100% abortion. Likewise, IFNγ levels in G1 increased earlier than those in other intravenously infected groups, and IgG levels on day 21 post-infection (pi) were higher in G1 than those in other intravenously infected groups. Concerning vertical transmission, G1 shows a higher parasite burden in the foetal brain than did G2 and G3. Comparing routes of administration, no differences in foetal survival rate or parasite load in the foetal brain were found. Although G2 had higher IFNγ levels than G5 on day 10 pi, no differences were found in humoral immune responses. Because the outcome after intravenous infection with 10 5 tachyzoites was similar to that observed after intravenous infection with 10 6 tachyzoites used in a previous work (100% abortion and vertical transmission), we conclude that it may be reasonable to use 10 5 tachyzoites administered by the intravenous route in further experiments when assessing drugs or vaccine candidates. |
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