Isolation and sequence analysis of pCS36-4CPA, a small plasmid from Citrobacter sp. 36-4CPA.

Autor:	Zharikova NV; Ufa Institute of Biology, Russian Academy of Sciences, Russia., Iasakov TR; Ufa Institute of Biology, Russian Academy of Sciences, Russia., Bumazhkin BK; Bioengineering Center, Russian Academy of Sciences, Russia., Patutina EO; Bioengineering Center, Russian Academy of Sciences, Russia., Zhurenko EI; Ufa Institute of Biology, Russian Academy of Sciences, Russia., Korobov VV; Ufa Institute of Biology, Russian Academy of Sciences, Russia., Sagitova AI; Ufa Institute of Biology, Russian Academy of Sciences, Russia., Kuznetsov BB; Bioengineering Center, Russian Academy of Sciences, Russia., Markusheva TV; Ufa Institute of Biology, Russian Academy of Sciences, Russia.
Jazyk:	angličtina
Zdroj:	Saudi journal of biological sciences [Saudi J Biol Sci] 2018 May; Vol. 25 (4), pp. 660-671. Date of Electronic Publication: 2016 Feb 12.
DOI:	10.1016/j.sjbs.2016.02.014
Abstrakt:	A small plasmid designated pCS36-4CPA with a size of 5217 base pairs and G-C content of 50.74% was isolated from Citrobacter sp. 36-4CPA. The origin of replication ( ori ) of the plasmid was identified as a region of about 800 bp in length with an identity of 67.1% to the ColE1 plasmid at the nucleotide level. The replication region contained typical elements of ColE1-like plasmids: RNA I and RNA II with their corresponding -10 and -35 boxes, a single-strand initiation site ( ssi ), and a lagging-strand termination site ( terH ). As seen in other ColE1-like plasmids, pCS36-4CPA carried mobilisation machinery that include mobABCD genes but it did not possess the rom gene. Analysis of the multimer resolution site ( mrs ) was performed and XerC and XerD binding sites were identified. Also, the 70-nt transcript Rcd of pCS36-4CPA was predicted and similarity of the transcript's secondary structure with those of the ColE1-family was shown. The cargo module of pCS36-4CPA contained three open reading frames (ORFs). Two of them (ORF5 and ORF6) showed no significant homology to any known gene sequences but contained putative THAP DNA-binding (DBD) and type II restriction endonuclease Eco O109I domains. The seventh open reading frame (ORF7) encodes YhdJ-like DNA modification methylase. The region highly homologous to pCS36-4CPA was found in the Salmonella phage SE2 genome.
Databáze:	MEDLINE
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