| Autor: |
Huyen CTT; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. thanhhuyen0187@hotmail.com.; Hanoi Univerisity of Pharmacy, 13⁻15 Le Thanh Tong, Hanoi, Vietnam. thanhhuyen0187@hotmail.com., Luyen BTT; Hanoi Univerisity of Pharmacy, 13⁻15 Le Thanh Tong, Hanoi, Vietnam. luyenbthoaduoc@gmail.com., Khan GJ; State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering (BME), Southeast University, Nanjing 210096, China. U4574904@hotmail.com., Oanh HV; Hanoi Univerisity of Pharmacy, 13⁻15 Le Thanh Tong, Hanoi, Vietnam. havanoanh@gmail.com., Hung TM; National Institute of Drug Quality Control (NIDQC), 48 Hai Ba Trung, Hoan Kiem, Hanoi, Vietnam. tahungvkn@gmail.com., Li HJ; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. cpuli@163.com., Li P; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. liping@cpu.edu.cn. |
| Abstrakt: |
This study was designed to search for novel anti-cancer compounds from natural plants. The 70% ethanolic extract from the rizhomes of Cimicifuga dahurica (Turcz.) Maxim. (Ranunculaceae) was found to possess significant in vitro anti-proliferative effects on MCF-7 breast cancer cells. A phytochemical investigation using assay-guided fractionation of the ethanolic extract of C. dahurica resulted in the isolation of one new phenolic amide glycoside 3 , two new lignan glycosides 4 and 7 , one new 9,19-cycloartane triterpenoid glycoside 6 , and thirteen known constituents 1 , 2 , 5 , and 8 ⁻ 17 . The structures of 3 , 4 , 6 , and 7 were established using contemporary NMR methods and from their HRESIMS data. The anti-proliferative effects of isolated compounds were evaluated using the BrdU-proliferation kit. Five among the 17 isolated compounds showed significant anti-proliferative effects ( p ≤ 0.05), wherein compound 7 showed the most significant anti-proliferative and cell cycle arresting effect ( p ≤ 0.05) which followed a dose dependent manner. Western blot protein expression analysis showed a down expression of c-Myc and cyclin D1 which further elucidated the anti-proliferation mechanism of compound 7 while apoptotic effects were found in association with Bcl-2 family protein expression variations. Conclusively this study reports the isolation and identification of 17 compounds from C. dahurica , including four novel molecules, in addition to the fact that compound 7 possesses significant anti-proliferative and apoptotic effects in vitro that may require further exploration. |
