Ebselen protects mitochondrial function and oxidative stress while inhibiting the mitochondrial apoptosis pathway after acute spinal cord injury.

Autor: Jia ZQ; Department of Orthopedics, The Second Affiliated Hospital of Xi'an Jiaotong University, Xian 710004, PR China; Department of Spinal Surgery, The Second Affiliated Hospital of Henan University of Science and Technology, Luoyang 471000, PR China. Electronic address: jzqdoctor@163.com., Li SQ; Medical College, Henan University of Science and Technology, Luoyang 471003, PR China., Qiao WQ; Department of Breast Surgery, The First Affiliated Hospital, College of Clinical Medicine of Henan University of Science and Technology, Luoyang, PR China., Xu WZ; Department of Spinal Surgery, The Second Affiliated Hospital of Henan University of Science and Technology, Luoyang 471000, PR China., Xing JW; Department of Spinal Surgery, The Second Affiliated Hospital of Henan University of Science and Technology, Luoyang 471000, PR China., Liu JT; Department of Orthopedics, The Second Affiliated Hospital of Xi'an Jiaotong University, Xian 710004, PR China., Song H; Department of Orthopedics, The Second Affiliated Hospital of Xi'an Jiaotong University, Xian 710004, PR China., Gao ZY; Department of Orthopedics, The Second Affiliated Hospital of Xi'an Jiaotong University, Xian 710004, PR China., Xing BW; Medical College, Henan University of Science and Technology, Luoyang 471003, PR China., He XJ; Department of Orthopedics, The Second Affiliated Hospital of Xi'an Jiaotong University, Xian 710004, PR China. Electronic address: xijinghe2017@163.com.
Jazyk: angličtina
Zdroj: Neuroscience letters [Neurosci Lett] 2018 Jun 21; Vol. 678, pp. 110-117. Date of Electronic Publication: 2018 May 04.
DOI: 10.1016/j.neulet.2018.05.007
Abstrakt: Ebselen is a fat-soluble small molecule and organic selenium compound that regulates the activity of glutathione peroxidase to alleviate mitochondrial oxidative stress and improve mitochondrial function. In the present study, we aimed to investigate the effects of ebselen on mitochondrial oxidative stress response, mitochondrial apotosis, and motor behaviors after spinal cord injury (SCI). We found that ebselen significantly increased the BBB score in motor behavior, thus suggesting a rescue effect of ebselen on motor function after SCI in rats. Meanwhile, we revealed that ebselen can increase glutathione (GSH) content as well as superoxide dismutase (SOD) and catalase (CAT) activities after SCI-this suggests ebselen has an antioxidant effect. Furthermore, the ATP content and Na + -K + -ATPase activity in mitochondria were increased by ebselen after SCI, while the mitochondrial membrane potential (MMP) was decreased by ebselen. The Cytochrome C and Smac release from mitochondria were reduced by ebselen after SCI, thus indicating improved membrane permeability by ebselen. Moreover, the alterations in caspase-3, Bax and Bcl-2 protein expression, as well as the proportion of cell apoptosis were improved by ebselen treatment, which together suggested that ebselen has an inhibitory effect on mitochondrial apotosis pathways after SCI. Taken together, our results suggest that ebselen can inhibit secondary damage caused by spinal cord injury. Indeed it plays a neuroprotective role in spinal cord injury perhaps by improving mitochondrial function and inhibiting the mitochondrial apoptosis pathway.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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