RUNX1 promotes cell growth in human T-cell acute lymphoblastic leukemia by transcriptional regulation of key target genes.

Autor: Jenkins CE; Terry Fox Laboratory/Department of Pathology, BC Cancer Agency, Vancouver, BC, Canada., Gusscott S; Terry Fox Laboratory/Department of Pathology, BC Cancer Agency, Vancouver, BC, Canada., Wong RJ; Terry Fox Laboratory/Department of Pathology, BC Cancer Agency, Vancouver, BC, Canada., Shevchuk OO; Terry Fox Laboratory/Department of Pathology, BC Cancer Agency, Vancouver, BC, Canada., Rana G; Terry Fox Laboratory/Department of Pathology, BC Cancer Agency, Vancouver, BC, Canada., Giambra V; Terry Fox Laboratory/Department of Pathology, BC Cancer Agency, Vancouver, BC, Canada., Tyshchenko K; Terry Fox Laboratory/Department of Pathology, BC Cancer Agency, Vancouver, BC, Canada., Islam R; Department of Microbiology & Immunology, Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada; Canada's Michael Smith Genome Sciences Centre, BC Cancer Agency, Vancouver, BC, Canada., Hirst M; Department of Microbiology & Immunology, Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada; Canada's Michael Smith Genome Sciences Centre, BC Cancer Agency, Vancouver, BC, Canada., Weng AP; Terry Fox Laboratory/Department of Pathology, BC Cancer Agency, Vancouver, BC, Canada. Electronic address: aweng@bccrc.ca.
Jazyk: angličtina
Zdroj: Experimental hematology [Exp Hematol] 2018 Aug; Vol. 64, pp. 84-96. Date of Electronic Publication: 2018 May 05.
DOI: 10.1016/j.exphem.2018.04.008
Abstrakt: RUNX1 is frequently mutated in T-cell acute lymphoblastic leukemia (T-ALL). The spectrum of RUNX1 mutations has led to the notion that it acts as a tumor suppressor in this context; however, other studies have placed RUNX1, along with transcription factors TAL1 and NOTCH1, as core drivers of an oncogenic transcriptional program. To reconcile these divergent roles, we knocked down RUNX1 in human T-ALL cell lines and deleted Runx1 or Cbfb in primary mouse T-cell leukemias. RUNX1 depletion consistently resulted in reduced cell proliferation and increased apoptosis. RUNX1 upregulated variable sets of target genes in each cell line, but consistently included a core set of oncogenic effectors including insulin-like growth factor 1 receptor (IGF1R) and NRAS. Our results support the conclusion that RUNX1 has a net positive effect on cell growth in the context of established T-ALL.
(Crown Copyright © 2018. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: