Loss of inter-cellular cooperation by complete epithelial-mesenchymal transition supports favorable outcomes in basal breast cancer patients.

Autor:	Grosse-Wilde A; Institute for Systems Biology, Seattle, WA, USA., Kuestner RE; Institute for Systems Biology, Seattle, WA, USA., Skelton SM; Institute for Systems Biology, Seattle, WA, USA., MacIntosh E; Institute for Systems Biology, Seattle, WA, USA., d'Hérouël AF; Institute for Systems Biology, Seattle, WA, USA.; Luxembourg Centre for Systems Biomedicine, Esch-sur-Alzette, Luxembourg., Ertaylan G; Luxembourg Centre for Systems Biomedicine, Esch-sur-Alzette, Luxembourg.; Environmental Risk and Health Unit, Flemish Institute for Technological Research (VITO), Mol, Belgium., Del Sol A; Luxembourg Centre for Systems Biomedicine, Esch-sur-Alzette, Luxembourg., Skupin A; Institute for Systems Biology, Seattle, WA, USA.; Luxembourg Centre for Systems Biomedicine, Esch-sur-Alzette, Luxembourg., Huang S; Institute for Systems Biology, Seattle, WA, USA.
Jazyk:	angličtina
Zdroj:	Oncotarget [Oncotarget] 2018 Apr 13; Vol. 9 (28), pp. 20018-20033. Date of Electronic Publication: 2018 Apr 13 (Print Publication: 2018).
DOI:	10.18632/oncotarget.25034
Abstrakt:	According to the sequential metastasis model, aggressive mesenchymal (M) metastasis-initiating cells (MICs) are generated by an epithelial-mesenchymal transition (EMT) which eventually is reversed by a mesenchymal-epithelial transition (MET) and outgrowth of life-threatening epithelial (E) macrometastases. Paradoxically, in breast cancer M signatures are linked with more favorable outcomes than E signatures, and M cells are often dispensable for metastasis in mouse models. Here we present evidence at the cellular and patient level for the cooperation metastasis model, according to which E cells are MICs, while M cells merely support E cell persistence through cooperation. We tracked the fates of co-cultured E and M clones and of fluorescent CDH1-promoter-driven cell lines reporting the E state derived from basal breast cancer HMLER cells. Cells were placed in suspension state and allowed to reattach and select an EMT cell fate. Flow cytometry, single cell and bulk gene expression analyses revealed that only pre-existing E cells generated E cells, mixed E/M populations, or stem-like hybrid E/M cells after suspension and that complete EMT manifest in M clones and CDH1-negative reporter cells resulted in loss of cell plasticity, suggesting full transdifferentiation. Mechanistically, E-M coculture experiments supported the persistence of pre-existing E cells where M cells inhibited EMT of E cells in a mutual cooperation via direct cell-cell contact. Consistently, M signatures were associated with more favorable patient outcomes compared to E signatures in breast cancer, specifically in basal breast cancer patients. These findings suggest a potential benefit of complete EMT for basal breast cancer patients. Competing Interests: CONFLICTS OF INTEREST The authors declare no financial and non-financial-competing interests.
Databáze:	MEDLINE
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