Increased plasmatic soluble HLA-G levels in endometrial cancer.

Autor: Ben Yahia H; Laboratoire Microorganismes et Biomolécules Actives, Département de Biologie, Faculté des Sciences de Tunis, Université Tunis El Manar, 2092, Tunis, Tunisia., Babay W; Laboratoire Microorganismes et Biomolécules Actives, Département de Biologie, Faculté des Sciences de Tunis, Université Tunis El Manar, 2092, Tunis, Tunisia., Bortolotti D; Department of Experimental and Diagnostic Medicine, Section Microbiology, University of Ferrara, Ferrara, Italy., Boujelbene N; Department of Pathology, Salah Azaïz Institute, Tunis, Tunisia., Laaribi AB; Laboratoire Microorganismes et Biomolécules Actives, Département de Biologie, Faculté des Sciences de Tunis, Université Tunis El Manar, 2092, Tunis, Tunisia., Zidi N; Department of Medical Oncology, Salah Azaïz Institute, Tunis, Tunisia., Kehila M; Department of Obstetrics and Gynecology, Tunis Maternity and Neonatology Center, Tunis El Manar University, Tunisia., Chelbi H; Laboratory of Medical Parasitology, Biotechnology and Biomolecules, Pasteur Institute of Tunis, Tunisia., Boudabous A; Laboratoire Microorganismes et Biomolécules Actives, Département de Biologie, Faculté des Sciences de Tunis, Université Tunis El Manar, 2092, Tunis, Tunisia., Mrad K; Department of Pathology, Salah Azaïz Institute, Tunis, Tunisia., Mezlini A; Department of Medical Oncology, Salah Azaïz Institute, Tunis, Tunisia., Di Luca D; Department of Experimental and Diagnostic Medicine, Section Microbiology, University of Ferrara, Ferrara, Italy., Ouzari HI; Laboratoire Microorganismes et Biomolécules Actives, Département de Biologie, Faculté des Sciences de Tunis, Université Tunis El Manar, 2092, Tunis, Tunisia., Rizzo R; Department of Experimental and Diagnostic Medicine, Section Microbiology, University of Ferrara, Ferrara, Italy., Zidi I; Laboratoire Microorganismes et Biomolécules Actives, Département de Biologie, Faculté des Sciences de Tunis, Université Tunis El Manar, 2092, Tunis, Tunisia. Electronic address: ines.zidi@istmt.utm.tn.
Jazyk: angličtina
Zdroj: Molecular immunology [Mol Immunol] 2018 Jul; Vol. 99, pp. 82-86. Date of Electronic Publication: 2018 May 03.
DOI: 10.1016/j.molimm.2018.04.007
Abstrakt: Human Leukocyte Antigen-G (HLA-G) is known as an immune suppressive molecule; it interacts with several immune cells and inhibits their functions. HLA-G molecule is highly represented in pathological conditions including malignant transformation. To the best of our knowledge this is the first study that focuses on the expression of soluble HLA-G (sHLA-G) in endometrial cancer (EC). We aimed at exploring sHLA-G plasma levels and its prognostic value in EC. We examined total sHLA-G expression as well as the sHLA-G1 and HLA-G5 isoforms expression in plasma samples from 40 patients with EC and 45 healthy controls by a specific sandwich ELISA. Immunoprecipitation and Coomassie blue staining were performed to explore the presence of plasmatic sHLA-G monomers and dimers. sHLA-G plasma level was significantly enhanced in patients with EC compared to healthy controls (p = 0.028). Additionally, HLA-G5 molecules were highly represented than sHLA-G1 molecules in EC, at the borderline of significance (p = 0.061). Interestingly, sHLA-G has been shown to be increased in early stages (Stages I and II) as well as in high grade EC (Grade 3) that is associated with rapid spread of the disease (p = 0.057). sHLA-G positive EC plasma were majorly in monomeric form (75%). Clinically, all the HLA-G dimers were detected in early stages and in high grade of EC. Our data strengthen the implication of HLA-G molecules in EC etiology and especially in progression.
(Copyright © 2018 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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