Millettia macrophylla (Fabaceae) phenolic fraction prevents differentiation of 3T3-L1 adipocytes and the increased risks of cardiovascular diseases in ovariectomized rats.

Autor: Zingue S; Department of Life and Earth Sciences, Higher Teachers' Training College, University of Maroua, P.O. Box 55, Maroua, Cameroon; Department of Pharmaceutical Sciences, Health Sciences Centre, Federal University of Santa Catarina, CEP 88040-900 Florianópolis, Santa Catarina, Brazil; Division of Pharmacognosy and Natural Products Chemistry, School of Pharmacy, University of Athens, Panepistimioupoli Zografou, 15771 Athens, Greece. Electronic address: stephanezingue@gmail.com., do Carmo ÍAR; Department of Clinical Analysis, Health Sciences Centre, Federal University of Santa Catarina, CEP 88040-900 Florianópolis, Santa Catarina, Brazil. Electronic address: icaro.ar@gmail.com., Tchoumtchoua J; Division of Pharmacognosy and Natural Products Chemistry, School of Pharmacy, University of Athens, Panepistimioupoli Zografou, 15771 Athens, Greece. Electronic address: tchoumtchoua@gmail.com., Tchoupang EN; Department of Animal Biology and Physiology, Faculty of Sciences, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon. Electronic address: tchoupangnanaedwige@yahoo.fr., Bratti LOS; Department of Clinical Analysis, Health Sciences Centre, Federal University of Santa Catarina, CEP 88040-900 Florianópolis, Santa Catarina, Brazil. Electronic address: leticiaosbratti@gmail.com., Mora TD; Department of Clinical Analysis, Health Sciences Centre, Federal University of Santa Catarina, CEP 88040-900 Florianópolis, Santa Catarina, Brazil. Electronic address: tamaradalmora@hotmail.com., Halabalaki M; Division of Pharmacognosy and Natural Products Chemistry, School of Pharmacy, University of Athens, Panepistimioupoli Zografou, 15771 Athens, Greece. Electronic address: mariahal@pharm.uoa.gr., Njamen D; Department of Animal Biology and Physiology, Faculty of Sciences, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon. Electronic address: dnjamen@gmail.com., Creczynski-Pasa TB; Department of Pharmaceutical Sciences, Health Sciences Centre, Federal University of Santa Catarina, CEP 88040-900 Florianópolis, Santa Catarina, Brazil. Electronic address: tania.pasa@ufsc.br., Filippin-Monteiro FB; Department of Clinical Analysis, Health Sciences Centre, Federal University of Santa Catarina, CEP 88040-900 Florianópolis, Santa Catarina, Brazil. Electronic address: fabiola.monteiro@ufsc.br.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2018 Aug 10; Vol. 222, pp. 87-98. Date of Electronic Publication: 2018 May 01.
DOI: 10.1016/j.jep.2018.04.046
Abstrakt: Ethnopharmacological Relevance: A prolonged estrogen deficiency alters lipid metabolism and increases risks of cardiovascular diseases. Phytoestrogens, naturally occurring compounds with estrogenic properties are reported to have cardiovascular protective effects. Millettia macrophylla used in the Cameroonian traditional system to treat physiological disorders related to menopause, was previously reported to have estrogenic effects.
Aim: We, therefore, proposed evaluating the in vitro and in vivo effects of M. macrophylla phenolic fraction on some risk factors for cardiovascular diseases.
Material and Methods: In vitro, the ability of the M. macrophylla phenolic fraction (PF) as well as the 9 isolates to prevent the 3T3-L1 preadipocytes differentiation was assessed. Further, the preventive effects of PF on abdominal fat accumulation, body weight gain, lipid profile, nitric oxide level, superoxide dismutase (SOD) and catalase activities, reduced glutathione (GSH) and malondialdehyde (MDA) levels were assessed in a postmenopausal rat model.
Results: In vitro, PF and its isolate secundiferol I inhibited lipid accumulation in 3T3-L1 cells. Moreover, all the isolates except daidzein dimethylether prevented the interleukin IL-6 production in 3T3-L1 cells. In vivo, PF prevented ovariectomy-induced abdominal fat accumulation, body weight gain, dyslipidemia, glucose intolerance and decreased atherogenic index. In addition, it induced a vasorelaxant effect by preventing the low level of nitric oxide in the aorta. PF also exhibited antioxidant effects as it increased aorta GSH level, SOD, and catalase activities and decreased MDA level.
Conclusions: Taken together, our data suggest that PF prevents the increased risks of cardiovascular diseases in ovariectomized rats.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: