Demethoxycurcumin Suppresses Migration and Invasion of Human Cervical Cancer HeLa Cells via Inhibition of NF-κB Pathways.

Autor: Lin CC; Departments of Chinese Medicine, Feng-Yuan Hospital, Ministry of Health and Welfare, Executive Yuan, Taichung, Taiwan, R.O.C.; General Education Center, Central Taiwan University of Science and Technology, Taichung, Taiwan, R.O.C., Kuo CL; Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, Taiwan, R.O.C., Huang YP; Department of Physiology, China Medical University, Taichung, Taiwan, R.O.C., Chen CY; Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C., Hsu MJ; Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C., Chu YL; International Master's Degree Program in Food Science, International College, National Pingtung University of Science and Technology, Pingtung, Taiwan, R.O.C., Chueh FS; Department of Food Nutrition and Health Biotechnology, Asia University, Taichung, Taiwan, R.O.C. jgchung@mail.cmu.edu.tw fushin@asia.edu.tw., Chung JG; Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C. jgchung@mail.cmu.edu.tw fushin@asia.edu.tw.; Department of Biotechnology, Asia University, Taichung, Taiwan, R.O.C.
Jazyk: angličtina
Zdroj: Anticancer research [Anticancer Res] 2018 May; Vol. 38 (5), pp. 2761-2769.
DOI: 10.21873/anticanres.12519
Abstrakt: Background/aim: Demethoxycurcumin (DMC), one of the curcuminoids present in turmeric, has been shown to induce cell death in many human cancer cell lines, however, there has not been any investigation on whether DMC inhibits metastatic activity in human cervical cancer cells in vitro. In the present study, DMC at 2.5-15 μM decreased cell number, thus, we used IC 20 (7.5 μM) for further investigation of its anti-metastatic activity in human cervical cancer HeLa cells.
Materials and Methods: The wound healing, migration, invasion, zymography, and western blotting assays were used to investigate the effects of DMC on HeLa cells.
Results: The wound healing assay was used to show that DMC suppressed cell movement of HeLa cells. Furthermore, the trans-well chamber assay was used to show that DMC suppressed HeLa cell migration and invasion. Gelatin zymography assay did not show any significant effects of DMC on the gelatinolytic activity (MMP-2 and -9) in conditioned media of HeLa cells treated by DMC. Western blotting showed that DMC significantly reduced protein levels of GRB2, MMP-2, ERK1/2, N-cadherin and Ras but increased the levels of E-cadherin and NF-κB in HeLa cells. Confocal laser microscopy indicated that DMC increased NF-κB in HeLa cells confirming the results from Western blotting.
Conclusion: DMC may be used as a novel anti-metastatic agent for the treatment of human cervical cancer in the future.
(Copyright© 2018, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
Databáze: MEDLINE
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