Hsa_circ_0071589 promotes carcinogenesis via the miR-600/EZH2 axis in colorectal cancer.

Autor: Yong W; Department of Colorectal Surgery, The First Affiliated Hospital of Nanjing Medical University, 210029, Nanjing City, Jiangsu Province, China., Zhuoqi X; Department of Colorectal Surgery, The First Affiliated Hospital of Nanjing Medical University, 210029, Nanjing City, Jiangsu Province, China., Baocheng W; Department of Colorectal Surgery, The First Affiliated Hospital of Nanjing Medical University, 210029, Nanjing City, Jiangsu Province, China., Dongsheng Z; Department of Colorectal Surgery, The First Affiliated Hospital of Nanjing Medical University, 210029, Nanjing City, Jiangsu Province, China., Chuan Z; Department of Colorectal Surgery, The First Affiliated Hospital of Nanjing Medical University, 210029, Nanjing City, Jiangsu Province, China., Yueming S; Department of Colorectal Surgery, The First Affiliated Hospital of Nanjing Medical University, 210029, Nanjing City, Jiangsu Province, China. Electronic address: sunym_njedu@163.com.
Jazyk: angličtina
Zdroj: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2018 Jun; Vol. 102, pp. 1188-1194. Date of Electronic Publication: 2018 Apr 10.
DOI: 10.1016/j.biopha.2018.03.085
Abstrakt: Previous studies have revealed that miRNAs and lncRNAs participate in the pathogenesis of colorectal cancer (CRC); however, whether circular RNAs (circRNAs) are also involved remains unclear. In the present study, qRT-PCR was used to examine the expression of hsa_circ_0071589, miR-600, and enhancer of zeste homolog 2 (EZH2) in CRC. MTT assay, colony formation assay, transwell assay, and wound-healing assay were performed to assess the effects of hsa_circ_0071589, miR-600, and EZH2 on CRC cell viability, proliferation, invasion, and migration. Bioinformatics analysis, luciferase reporter assay, and RIP assay were used to explore the correlations among hsa_circ_0071589, miR-600, and EZH2 expression in CRC cells. The results showed that hsa_circ_0071589 expression was significantly higher in CRC tissues than in normal tissues. Blockage of hsa_circ_0071589 in CRC cells inhibited tumor growth, invasion and migration. Hsa_circ_0071589 was able to promote the expression of EZH2 by acting as a sponge of miR-600. In addition, miR-600 expression was negatively correlated to hsa_circ_0071589 expression in CRC tissues. These results demonstrated that the hsa_circ_0071589/miR-600/EZH2 axis may play critical regulatory roles in the pathogenesis of CRC and may serve as a novel therapy target in CRC.
(Copyright © 2018 Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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