| Autor: |
Gao J; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory; Joint Genome Institute, Department of Energy., Sasse J; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory; Joint Genome Institute, Department of Energy., Lewald KM; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory; Joint Genome Institute, Department of Energy., Zhalnina K; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory; Joint Genome Institute, Department of Energy., Cornmesser LT; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory; Joint Genome Institute, Department of Energy., Duncombe TA; Joint BioEnergy Institute., Yoshikuni Y; Joint Genome Institute, Department of Energy., Vogel JP; Joint Genome Institute, Department of Energy., Firestone MK; Department of Environmental Science Policy and Management, University of California., Northen TR; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory; Joint Genome Institute, Department of Energy; trnorthen@lbl.gov. |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of visualized experiments : JoVE [J Vis Exp] 2018 Apr 10 (134). Date of Electronic Publication: 2018 Apr 10. |
| DOI: |
10.3791/57170 |
| Abstrakt: |
Beneficial plant-microbe interactions offer a sustainable biological solution with the potential to boost low-input food and bioenergy production. A better mechanistic understanding of these complex plant-microbe interactions will be crucial to improving plant production as well as performing basic ecological studies investigating plant-soil-microbe interactions. Here, a detailed description for ecosystem fabrication is presented, using widely available 3D printing technologies, to create controlled laboratory habitats (EcoFABs) for mechanistic studies of plant-microbe interactions within specific environmental conditions. Two sizes of EcoFABs are described that are suited for the investigation of microbial interactions with various plant species, including Arabidopsis thaliana, Brachypodium distachyon, and Panicum virgatum. These flow-through devices allow for controlled manipulation and sampling of root microbiomes, root chemistry as well as imaging of root morphology and microbial localization. This protocol includes the details for maintaining sterile conditions inside EcoFABs and mounting independent LED light systems onto EcoFABs. Detailed methods for addition of different forms of media, including soils, sand, and liquid growth media coupled to the characterization of these systems using imaging and metabolomics are described. Together, these systems enable dynamic and detailed investigation of plant and plant-microbial consortia including the manipulation of microbiome composition (including mutants), the monitoring of plant growth, root morphology, exudate composition, and microbial localization under controlled environmental conditions. We anticipate that these detailed protocols will serve as an important starting point for other researchers, ideally helping create standardized experimental systems for investigating plant-microbe interactions. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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