Mineralization of gellan gum hydrogels with calcium and magnesium carbonates by alternate soaking in solutions of calcium/magnesium and carbonate ion solutions.

Autor: Lopez-Heredia MA; Univ. Lille, Inserm, CHU Lille, U1008 - Controlled Drug Delivery Systems and Biomaterials, Lille, France., Łapa A; Department of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Kraków, Poland., Reczyńska K; Department of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Kraków, Poland., Pietryga K; Department of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Kraków, Poland., Balcaen L; Department of Analytical Chemistry, Ghent University, Ghent, Belgium., Mendes AC; Nano-BioScience Research Group, DTU-Food, Technical University of Denmark (DTU), Kongens Lyngby, Denmark., Schaubroeck D; Centre for Microsystems Technology (CMST), imec, Ghent University, Ghent, Belgium., Van Der Voort P; Department of Inorganic Chemistry, COMOC, Ghent University, Ghent, Belgium., Dokupil A; Institute for Chemical Processing of Coal (ICHPW), Zabrze, Poland., Plis A; Institute for Chemical Processing of Coal (ICHPW), Zabrze, Poland., Stevens CV; Department of Sustainable Organic Chemistry and Technology, Ghent University, Ghent, Belgium., Parakhonskiy BV; Department Molecular Biotechology, Ghent University, Ghent, Belgium.; Shubnikov Institute of Crystallography, FSRC 'Crystallography and Photonics' RAS, Moscow, Russia., Samal SK; Laboratory of General Biochemistry and Physical Pharmacy, Ghent University, Ghent, Belgium.; Centre for Nano- and Biophotonics, Ghent University, Ghent, Belgium., Vanhaecke F; Department of Analytical Chemistry, Ghent University, Ghent, Belgium., Chai F; Univ. Lille, Inserm, CHU Lille, U1008 - Controlled Drug Delivery Systems and Biomaterials, Lille, France., Chronakis IS; Nano-BioScience Research Group, DTU-Food, Technical University of Denmark (DTU), Kongens Lyngby, Denmark., Blanchemain N; Univ. Lille, Inserm, CHU Lille, U1008 - Controlled Drug Delivery Systems and Biomaterials, Lille, France., Pamuła E; Department of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Kraków, Poland., Skirtach AG; Department Molecular Biotechology, Ghent University, Ghent, Belgium.; Centre for Nano- and Biophotonics, Ghent University, Ghent, Belgium., Douglas TEL; Department Molecular Biotechology, Ghent University, Ghent, Belgium.; Engineering Department, Lancaster University, Lancaster, UK.; Materials Science Institute (MSI), Lancaster University, Lancaster, UK.
Jazyk: angličtina
Zdroj: Journal of tissue engineering and regenerative medicine [J Tissue Eng Regen Med] 2018 Aug; Vol. 12 (8), pp. 1825-1834. Date of Electronic Publication: 2018 Jun 27.
DOI: 10.1002/term.2675
Abstrakt: Mineralization of hydrogels is desirable prior to applications in bone regeneration. CaCO 3 is a widely used bone regeneration material, and Mg, when used as a component of calcium phosphate biomaterials, has promoted bone-forming cell adhesion and proliferation and bone regeneration. In this study, gellan gum hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution. This alternate soaking cycle was repeated five times. Five different calcium and/or magnesium ion solutions, containing different molar ratios of Ca to Mg ranging from Mg free to Ca free were compared. Carbonate mineral formed in all sample groups subjected to the alternate soaking cycle. Ca : Mg elemental ratio in the mineral formed was higher than in the respective mineralizing solution. Mineral formed in the absence of Mg was predominantly CaCO 3 in the form of a mixture of calcite and vaterite. Increasing the Mg content in the mineral formed led to the formation of magnesian calcite and decreased the total amount of the mineral formed and its crystallinity. Hydrogel mineralization and increasing Mg content in mineral formed did not obviously improve proliferation of MC3T3-E1 osteoblast-like cells or differentiation after 7 days.
(© 2018 John Wiley & Sons, Ltd.)
Databáze: MEDLINE
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