ABO Antigens Active Tri- and Disaccharides Microarray to Evaluate C-type Lectin Receptor Binding Preferences.

Autor: Shanthamurthy CD; Indian Institute of Science Education and Research, Dr. Homi Bhabha Road, Pune, 411008, India., Jain P; Indian Institute of Science Education and Research, Dr. Homi Bhabha Road, Pune, 411008, India., Yehuda S; Tel-Aviv University, Department of Cell Research and Immunology, The George S. Wise Faculty of Life Sciences, Tel-Aviv, 69978, Israel., Monteiro JT; University of Veterinary Medicine Hannover, Immunology Unit & Research Center for Emerging Infections and Zoonoses, Hannover, Germany., Leviatan Ben-Arye S; Tel-Aviv University, Department of Cell Research and Immunology, The George S. Wise Faculty of Life Sciences, Tel-Aviv, 69978, Israel., Subramani B; Indian Institute of Science Education and Research, Dr. Homi Bhabha Road, Pune, 411008, India., Lepenies B; University of Veterinary Medicine Hannover, Immunology Unit & Research Center for Emerging Infections and Zoonoses, Hannover, Germany. bernd.lepenies@tiho-hannover.de., Padler-Karavani V; Tel-Aviv University, Department of Cell Research and Immunology, The George S. Wise Faculty of Life Sciences, Tel-Aviv, 69978, Israel. vkaravani@post.tau.ac.il., Kikkeri R; Indian Institute of Science Education and Research, Dr. Homi Bhabha Road, Pune, 411008, India. rkikkeri@iiserpune.ac.in.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2018 Apr 26; Vol. 8 (1), pp. 6603. Date of Electronic Publication: 2018 Apr 26.
DOI: 10.1038/s41598-018-24333-y
Abstrakt: Understanding blood group antigen binding preferences for C-type lectin receptors holds promise for modulating immune responses, since several Gram-negative bacteria express blood group antigens as molecular mimicry to evade immune responses. Herein, we report the synthesis of ABO blood group antigen active tri and disaccharides to investigate the binding specificity with various C-type lectin receptors using glycan microarray. The results of binding preferences show that distinct glycosylation on the galactose and fucose motifs are key for C-type lectin receptor binding and that these interactions occur in a Ca 2+ -dependent fashion.
Databáze: MEDLINE
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