Effect of selenium and vitamin E on acrosome reaction in porcine spermatozoa.

Autor: Tareq KMA; Laboratory of Animal Biotechnology, Faculty of Agriculture, Interdisciplinary Graduate School of Science and Technology Shinshu University 8304 Minamiminowa-mura 399-4598 Nagano Japan., Akter QS; Laboratory of Animal Biotechnology, Faculty of Agriculture, Interdisciplinary Graduate School of Science and Technology Shinshu University 8304 Minamiminowa-mura 399-4598 Nagano Japan., Takagi Y; Laboratory of Animal Biotechnology, Faculty of Agriculture, Interdisciplinary Graduate School of Science and Technology Shinshu University 8304 Minamiminowa-mura 399-4598 Nagano Japan., Hamano KI; Laboratory of Animal Biotechnology, Faculty of Agriculture, Interdisciplinary Graduate School of Science and Technology Shinshu University 8304 Minamiminowa-mura 399-4598 Nagano Japan., Sawada T; The Sawada Women's Clinic Nagoya Reproduction Center 464-0819 Nagaya Japan., Tsujii H; Laboratory of Animal Biotechnology, Faculty of Agriculture, Interdisciplinary Graduate School of Science and Technology Shinshu University 8304 Minamiminowa-mura 399-4598 Nagano Japan.
Jazyk: angličtina
Zdroj: Reproductive medicine and biology [Reprod Med Biol] 2009 Nov 28; Vol. 9 (2), pp. 73-81. Date of Electronic Publication: 2009 Nov 28 (Print Publication: 2010).
DOI: 10.1007/s12522-009-0041-x
Abstrakt: Purpose: Selenium (Se) and vitamin E (Vit-E), as an integral part of antioxidant systems, play an important role in the motility and acrosome reaction (AR) of mammalian spermatozoa. The purpose of this study was to investigate the effect of Se and Vit-E on motility, viability, AR and accumulation of ammonia in the culture medium during different incubation periods in porcine sperm.
Methods: Sperm samples were washed, swum-up and incubated at 37°C for 1 and 3 h in Sp-TALP medium supplemented with sodium selenite (SS), seleon l-methionine (SeMet) and Vit-E in the presence or absence of ammonia. Sperm motility was determined on the basis of movement quality examined by phase microscopy. Viability and AR of spermatozoa were assessed by Hoechst 33258 and chlortetracycline (CTC) staining technique, and accumulation of ammonia was measured by the indophenol method. The incorporation of 14 C(U)-glucose was assessed with a liquid scintillation counter.
Results: In experiment 1, the sperm motility, viability, AR and incorporation of 14 C(U)-glucose increased significantly ( P < 0.05) in SS, SeMet and Vit-E (5, 5 μg/l and 1.0 mM, respectively) compared with the control. In experiment 2, treatment of the sperm with SeMet and SeMet + Vit-E in the presence of 300 μM ammonia also resulted in a significant increase ( P < 0.05) in the rate of motility, viability, AR and incorporation of 14 C(U)-glucose. In contrast, the accumulation of ammonia was reduced by SeMet and SeMet + Vit-E compared with the other treatments.
Conclusions: These findings indicate that SeMet and SeMet + Vit-E may play an important role in reducing the accumulation of ammonia and subsequently in increasing the rate of AR and the utilization of glucose in porcine spermatozoa.
Databáze: MEDLINE
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