| Autor: |
Oliveira LG; Laboratório de Diagnóstico Laboratorial e Microbiologia Clínica, Campus Centro Oeste Dona Lindu, Universidade Federal de São João del Rei, Divinópolis, Minas Gerais, Brazil E-mail: magnacpaiva@ufsj.edu.br., Ferreira LGR; Laboratório de Diagnóstico Laboratorial e Microbiologia Clínica, Campus Centro Oeste Dona Lindu, Universidade Federal de São João del Rei, Divinópolis, Minas Gerais, Brazil E-mail: magnacpaiva@ufsj.edu.br., Nascimento AMA; Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Reis MP; Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Dias MF; Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Lima WG; Laboratório de Microbiologia Médica, Campus Centro Oeste Dona Lindu, Universidade Federal de São João del Rei, Divinópolis, Minas Gerais, Brazil., Paiva MC; Laboratório de Diagnóstico Laboratorial e Microbiologia Clínica, Campus Centro Oeste Dona Lindu, Universidade Federal de São João del Rei, Divinópolis, Minas Gerais, Brazil E-mail: magnacpaiva@ufsj.edu.br. |
| Abstrakt: |
Wastewater treatment plants (WWTPs) represent an important reservoir of antibiotic resistance determinants. Although many studies have been conducted to evaluate resistance profiles in Enterobacteriaceae isolates from this setting, the dynamics of this phenomenon are poorly known to the bacterium Pseudomonas aeruginosa. Here we aimed to evaluate the resistance profiles and the production of AmpC β-lactamase in P. aeruginosa isolates from a domestic full-scale WWTP. Samples of the raw sewage and effluent were collected and the bacterium P. aeruginosa was isolated on cetrimide agar. Susceptibility to β-lactams, fluoroquinolones and aminoglycosides was evaluated by the disc diffusion method, and the presence of AmpC β-lactamase was investigated phenotypically and by molecular method. We recovered 27 isolates of P. aeruginosa. Of these, 81.5% were susceptible to all antimicrobials tested. However, a considerable rate of resistance to carbapenems (11%) was found among the isolates. Twenty-two isolates were positive in the phenotypic test for inducible AmpC β-lactamase but the bla ampc gene was only identified in four isolates, suggesting the presence of other independent resistance mechanisms besides this β-lactamase. In summary, we have shown that P. aeruginosa isolates from a domestic WWTP represents a potential reservoir of bla ampC genes and other resistance determinants, including those that result in low susceptibility to carbapenems and aminoglycosides. |
