Comparison of two commercial embryo culture media (SAGE-1 step single medium vs. G1-PLUSTM/G2-PLUSTM sequential media): Influence on in vitro fertilization outcomes and human embryo quality.

Autor:	López-Pelayo I; Unit for Human Reproduction. UGC Clinical Laboratory. Puerta del Mar University Hospital. Cádiz. Spain., Gutiérrez-Romero JM; Unit for Human Reproduction. UGC Clinical Laboratory. Puerta del Mar University Hospital. Cádiz. Spain., Armada AIM; Unit for Human Reproduction. Comprenhensive Care for Woman. Puerta del Mar University Hospital. Cádiz. Spain., Calero-Ruiz MM; Unit for Human Reproduction. UGC Clinical Laboratory. Puerta del Mar University Hospital. Cádiz. Spain., Acevedo-Yagüe PJM; Unit for Human Reproduction. UGC Clinical Laboratory. Puerta del Mar University Hospital. Cádiz. Spain.
Jazyk:	angličtina
Zdroj:	JBRA assisted reproduction [JBRA Assist Reprod] 2018 Jun 01; Vol. 22 (2), pp. 128-133. Date of Electronic Publication: 2018 Jun 01.
DOI:	10.5935/1518-0557.20180024
Abstrakt:	Objective: To compare embryo quality, fertilization, implantation, miscarriage and clinical pregnancy rates for embryos cultured in two different commercial culture media until D-2 or D-3. Methods: In this retrospective study, we analyzed 189 cycles performed in 2016. Metaphase II oocytes were microinjected and allocated into single medium (SAGE 1-STEP, Origio) until transferred, frozen or discarded; or, if sequential media were used, the oocytes were cultured in G1-PLUSTM (Vitrolife) up to D-2 or D-3 and in G2-PLUSTM (Vitrolife) to transfer. On the following day, the oocytes were checked for normal fertilization and on D-2 and D-3 for morphological classification. Statistical analysis was performed using the chi-square and Mann-Whitney tests in PASW Statistics 18.0. Results: The fertilization rates were 70.07% for single and 69.11% for sequential media (p=0.736). The mean number of embryos with high morphological quality (class A/B) was higher in the single medium than in the sequential media: D-2 [class A (190 vs. 107, p<0.001), B (133 vs. 118, p=0.018)]; D-3 [class A (40 vs. 19, p=0.048) but without differences in class B (40 vs. 49)]. Consequently, a higher number of embryos cultured in single medium were frozen: 197 (21.00%) vs. sequential: 102 (11.00%), p<0.001. No differences were found in implantation rates (30.16% vs. 25.57%, p=0.520), clinical pregnancy rates (55.88% vs. 41.05%, p=0.213), or miscarriage rates (14.29% vs. 9.52%, p=0.472). Conclusion: Embryo culture in single medium yields greater efficiency per cycle than in sequential media. Higher embryo quality and quantity were achieved, resulting in more frozen embryos. There were no differences in clinical pregnancy rates.
Databáze:	MEDLINE
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