| Autor: |
Schmid FF; Translational Center Regenerative Therapies, Fraunhofer Institute for Silicate Research, Würzburg, Germany., Groeber-Becker F; Translational Center Regenerative Therapies, Fraunhofer Institute for Silicate Research, Würzburg, Germany. florian.kai.groeber-becker@isc.fraunhofer.de., Schwab S; Department of Cell and Tissue Engineering, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Stuttgart, Germany., Thude S; Department of Cell and Tissue Engineering, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Stuttgart, Germany., Walles H; Translational Center Regenerative Therapies, Fraunhofer Institute for Silicate Research, Würzburg, Germany.; Department Tissue Engineering & Regenerative Medicine, University Hospital Würzburg , Würzburg, Germany., Hansmann J; Translational Center Regenerative Therapies, Fraunhofer Institute for Silicate Research, Würzburg, Germany.; Department Tissue Engineering & Regenerative Medicine, University Hospital Würzburg , Würzburg, Germany. |
| Abstrakt: |
To protect the human skin from extensive solar radiation, melanocytes produce melanin and disperse it via melanosomes to keratinocytes in the basal and suprabasal layers of the human epidermis. Moreover, melanocytes are associated with pathological skin conditions such as vitiligo and psoriasis. Thus, an in vitro skin model that comprises a defined cutaneous pigmentation system is highly relevant in cosmetic, pharmaceutical and medical research. Here, we describe how the epidermal-melanin-unit can be established in vitro. Therefore, primary human melanocytes are implemented in an open source reconstructed epidermis. Following 14 days at the air liquid interface, a differentiated epidermis was formed and melanocytes were located in the basal layer. The functionality of the epidermal-melanin-unit could be shown by the transfer of melanin to the surrounding keratinocytes, and a significantly increased melanin content of models stimulated with either UV-radiation or the melanin precursor dihydroxyphenylalanine. Additionally, an UV50 assay was developed to test the protective effect of melanin. In analogy to the IC50 value in risk assessment, the UV50 value facilitates a quantitative investigation of harmful effects of natural UV-radiation to the skin in vitro. Employing this test, we could demonstrate that the melanin content correlates with the resilience against simulated sunlight, which comprises 2.5 % UVB and 97.5 % UVA. Besides demonstrating the protective effect of melanin in vitro, the assay was used to determine the protective effect of a consumer product in a highly standardized setup. |
