| Autor: |
Silva Rodrigues JF; Post-Graduation Program, Uniceuma University, são Luis 65075, MA, Brazil. joaofranciscosr@hotmail.com., Silva E Silva C; Post-Graduation Program, Uniceuma University, são Luis 65075, MA, Brazil. cristianeloud@gmail.com., França Muniz T; Post-Graduation Program, Uniceuma University, são Luis 65075, MA, Brazil. thayanne_muniz@hotmail.com., de Aquino AF; Post-Graduation Program, Uniceuma University, são Luis 65075, MA, Brazil. fernanda.aquino2@hotmail.com.; Florence Institute, Imunology Departament São Luis 65075, Brazil. fernanda.aquino2@hotmail.com., Neuza da Silva Nina L; Post-Graduation Program, Uniceuma University, são Luis 65075, MA, Brazil. l.snina.lnina@gmail.com., Fialho Sousa NC; Post-Graduation Program, Uniceuma University, são Luis 65075, MA, Brazil. nagila-caroline2011@live.com., Nascimento da Silva LC; Post-Graduation Program, Uniceuma University, são Luis 65075, MA, Brazil. luisclaudionsilva@yahoo.com.br., de Souza BGGF; Pathology Departament, State University of Maranhao, Sao Luis 65075, MA, Brazil. brenoggfs@hotmail.com., da Penha TA; Pathology Departament, State University of Maranhao, Sao Luis 65075, MA, Brazil. tatianearanha@hotmail.com., Abreu-Silva AL; Pathology Departament, State University of Maranhao, Sao Luis 65075, MA, Brazil. abreusilva.ana@gmail.com., de Sá JC; Post-Graduation Program, Uniceuma University, são Luis 65075, MA, Brazil. joicyvet@hotmail.com., Soares Fernandes E; Post-Graduation Program, Uniceuma University, são Luis 65075, MA, Brazil. lizbeth_fernandes@yahoo.co.uk., Grisotto MAG; Post-Graduation Program, Uniceuma University, são Luis 65075, MA, Brazil. marcos.grisotto@gmail.com.; Florence Institute, Imunology Departament São Luis 65075, Brazil. marcos.grisotto@gmail.com. |
| Abstrakt: |
Rheumatoid arthritis (RA) is characterized by inflammation of one or more joints, and affects ~1% of the adult population worldwide. Sulforaphane (SFN) is a natural compound that has been suggested as an antioxidant. Here, SFN’s effects were evaluated in a murine mono-arthritis model. Mono-arthritis was induced in mice by a single intra-articular injection of Complete Freund’s Adjuvant (CFA-10 µg/joint, in 10 µL) into the ipsilateral joint. The contralateral joint received an equal volume of PBS. On the 4th day post-joint inflammation induction, animals received either SFN (10 mg/kg) or vehicle (3% DMSO in saline), intraperitoneally (i.p.), twice a day for 3 days. Joint swelling and secondary mechanical allodynia and hyperalgesia were evaluated over 7 days post-CFA. After this period, animals were culled and their blood and synovial fluid samples were collected for analysis of cell populations, cytokine release and thioredoxin reductase (TrxR) activity. Knee joint samples were also collected for histology. SFN reduced joint swelling and damage whilst increasing the recruitment of Ly6C⁺ and Ly6G⁺ cells to CFA-injected joints. SFN-treated animals presented down-regulation of CD11b and CD62L on synovial fluid Ly6G⁺ cells. Synovial fluid samples obtained from CFA-injected joints and plasma samples of SFN-treated mice presented higher levels of IL-6 and increased activity of TrxR, in comparison with controls. These results indicate that SFN reduces knee joint damage by modulating cell activation/migration to the joints, cytokine production and increasing the activity of TrxR, and therefore, may represent an alternative treatment to joint inflammation. |
