Tissue remodeling after ocular surface reconstruction with denuded amniotic membrane.

Autor: Jie J; Eye Institute of Xiamen University, Xiamen, Fujian, China.; Medical College of Xiamen University, Xiamen, Fujian, China.; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Xiamen, Fujian, China.; Guilin Women and Children's Hospital, Guilin, Guangxi, China., Yang J; Eye Institute of Xiamen University, Xiamen, Fujian, China.; Medical College of Xiamen University, Xiamen, Fujian, China.; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Xiamen, Fujian, China.; Zhengzhou Second Hospital, Zhengzhou, Henan, China., He H; Eye Institute of Xiamen University, Xiamen, Fujian, China.; Medical College of Xiamen University, Xiamen, Fujian, China.; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Xiamen, Fujian, China., Zheng J; Xiamen University affiliated Chenggong Hospital, Xiamen, Fujian, China., Wang W; Xiamen University affiliated Chenggong Hospital, Xiamen, Fujian, China., Zhang L; Eye Institute of Xiamen University, Xiamen, Fujian, China.; Medical College of Xiamen University, Xiamen, Fujian, China.; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Xiamen, Fujian, China.; Xiamen University affiliated Xiamen Eye Center, Xiamen, Fujian, China., Li Z; Eye Institute of Xiamen University, Xiamen, Fujian, China.; Medical College of Xiamen University, Xiamen, Fujian, China.; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Xiamen, Fujian, China., Chen J; Eye Institute of Xiamen University, Xiamen, Fujian, China.; Medical College of Xiamen University, Xiamen, Fujian, China.; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Xiamen, Fujian, China., Vimalin Jeyalatha M; Eye Institute of Xiamen University, Xiamen, Fujian, China.; Medical College of Xiamen University, Xiamen, Fujian, China.; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Xiamen, Fujian, China., Dong N; Xiamen University affiliated Xiamen Eye Center, Xiamen, Fujian, China., Wu H; Xiamen University affiliated Xiamen Eye Center, Xiamen, Fujian, China., Liu Z; Eye Institute of Xiamen University, Xiamen, Fujian, China.; Medical College of Xiamen University, Xiamen, Fujian, China.; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Xiamen, Fujian, China.; Xiamen University affiliated Xiamen Eye Center, Xiamen, Fujian, China., Li W; Eye Institute of Xiamen University, Xiamen, Fujian, China. wei1018@xmu.edu.cn.; Medical College of Xiamen University, Xiamen, Fujian, China. wei1018@xmu.edu.cn.; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Xiamen, Fujian, China. wei1018@xmu.edu.cn.; The Affiliated Xiang'an Hospital of Xiamen University, Xiamen, Fujian, China. wei1018@xmu.edu.cn.; Xiamen University affiliated Xiamen Eye Center, Xiamen, Fujian, China. wei1018@xmu.edu.cn.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2018 Apr 23; Vol. 8 (1), pp. 6400. Date of Electronic Publication: 2018 Apr 23.
DOI: 10.1038/s41598-018-24694-4
Abstrakt: Amniotic membrane (AM) has been widely used as a temporary or permanent graft in the treatment of various ocular surface diseases. In this study, we compared the epithelial wound healing and tissue remodeling after ocular surface reconstruction with intact amniotic membrane (iAM) or denuded amniotic membrane (dAM). Partial limbal and bulbar conjunctival removal was performed on New Zealand rabbits followed by transplantation of cryo-preserved human iAM or dAM. In vivo observation showed that the epithelial ingrowth was faster on dAM compared to iAM after AM transplantation. Histological observation showed prominent epithelial stratification and increased goblet cell number on dAM after 2 weeks of follow up. Collagen VII degraded in dAM within 2 weeks, while remained in iAM even after 3 weeks. The number of macrophages and α-SMA positive cells in the stroma of remodelized conjunctiva in the dAM transplantation group was considerably less. In conclusion, dAM facilitates epithelial repopulation and goblet cell differentiation, further reduces inflammation and scar formation during conjunctival and corneal limbal reconstruction.
Databáze: MEDLINE