Identification of candidate genes mediating apple fruit-cracking resistance following the application of gibberellic acids 4 + 7 and the cytokinin 6-benzyladenine.

Autor: Joshi M; Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, 68 HaMaccabim Road, P.O. Box 15159, Rishon LeZion 7505101, Israel., Baghel RS; Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, 68 HaMaccabim Road, P.O. Box 15159, Rishon LeZion 7505101, Israel., Fogelman E; Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, 68 HaMaccabim Road, P.O. Box 15159, Rishon LeZion 7505101, Israel., Stern RA; MIGAL, Galilee Technology Center, P.O. Box 831, Kiryat Shmona 11016, Israel; Department of Biotechnology, Faculty of Life Sciences, Tel-Hai College, Upper Galilee 1220800, Israel., Ginzberg I; Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, 68 HaMaccabim Road, P.O. Box 15159, Rishon LeZion 7505101, Israel. Electronic address: iditgin@volcani.agri.gov.il.
Jazyk: angličtina
Zdroj: Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2018 Jun; Vol. 127, pp. 436-445. Date of Electronic Publication: 2018 Apr 14.
DOI: 10.1016/j.plaphy.2018.04.015
Abstrakt: Calyx-end cracking in 'Pink Lady' apple is treated by a solution of gibberellic acids 4 and 7 (GA 4+7 ) and the cytokinin 6-benzyladenine (BA). Although the GA 4+7 and BA mixture is applied early in apple fruit development, it mitigates cracking that becomes evident in the mature fruit, implying a long-term treatment effect. The reduced incidence of peel cracking is associated with increased epidermal cell density, which is maintained until fruit maturation. Presently, the expression of genes that have been previously reported to be associated with epidermal cell patterning and cuticle formation, or cracking resistance, was monitored in the peel during fruit development and following GA 4+7 and BA treatment. For most of the genes whose expression is naturally upregulated during fruit development, the early GA 4+7 and BA treatment maintained or further increased the high expression level in the mature peel. Where the expression of a gene was downregulated during development, no change was detected in the treated mature peel. Gene-networking analysis supported the interaction between gene clusters of cell-wall synthesis, cuticle formation and GA signaling. Overall, the data suggested that the GA 4+7 and BA treatment did not modify developmental cues, but promoted or enhanced the innate developmental program.
(Copyright © 2018 Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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