Draft genome sequence of field isolate Brucella melitensis strain 2007BM/1 from India.

Autor: Singh DK; Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izzatnagar, Bareilly 243122, Uttar Pradesh, India. Electronic address: dks@ivri.res.in., Kumar B; Division of Biological Products, ICAR-Indian Veterinary Research Institute, Izzatnagar, Bareilly 243122, Uttar Pradesh, India., Shrinet G; Division of Biological Products, ICAR-Indian Veterinary Research Institute, Izzatnagar, Bareilly 243122, Uttar Pradesh, India., Singh RP; Division of Biological Products, ICAR-Indian Veterinary Research Institute, Izzatnagar, Bareilly 243122, Uttar Pradesh, India., Das A; Division of Biological Products, ICAR-Indian Veterinary Research Institute, Izzatnagar, Bareilly 243122, Uttar Pradesh, India., Mantur BG; Department of Microbiology, Belgaum Institute of Medical Sciences, District Hospital Campus, Belgaum 590 001, Karnataka, India., Abhishek; Division of Veterinary Bacteriology and Mycology, ICAR-Indian Veterinary Research Institute, Izzatnagar, Bareilly 243122, Uttar Pradesh, India., Pandey A; Division of Veterinary Biotechnology, ICAR-Indian Veterinary Research Institute, Izzatnagar, Bareilly 243122, Uttar Pradesh, India., Mondal P; Division of Veterinary Biotechnology, ICAR-Indian Veterinary Research Institute, Izzatnagar, Bareilly 243122, Uttar Pradesh, India., Sajjanar BK; Division of Veterinary Biotechnology, ICAR-Indian Veterinary Research Institute, Izzatnagar, Bareilly 243122, Uttar Pradesh, India., Doimari S; Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izzatnagar, Bareilly 243122, Uttar Pradesh, India., Singh V; Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izzatnagar, Bareilly 243122, Uttar Pradesh, India., Kumari R; Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izzatnagar, Bareilly 243122, Uttar Pradesh, India., Tiwari AK; Division of Biological Standardization, ICAR-Indian Veterinary Research Institute, Izzatnagar, Bareilly 243122, Uttar Pradesh, India., Gandham RK; Division of Veterinary Biotechnology, ICAR-Indian Veterinary Research Institute, Izzatnagar, Bareilly 243122, Uttar Pradesh, India.
Jazyk: angličtina
Zdroj: Journal of global antimicrobial resistance [J Glob Antimicrob Resist] 2018 Jun; Vol. 13, pp. 152-153. Date of Electronic Publication: 2018 Apr 21.
DOI: 10.1016/j.jgar.2018.04.008
Abstrakt: Objectives: Brucellosis is among one of the most widespread important global zoonotic diseases that is endemic in many parts of India. Brucella melitensis is supposed to be the most pathogenic species for humans. Here we report the draft genome sequence of B. melitensis strain 2007BM/1 isolated from a human in India.
Methods: Genomic DNA was extracted from Brucella culture and was sequenced using an Illumina MiSeq platform. The generated reads were assembled using three de novo assemblers and the draft genome was annotated.
Results: This monoisolate, with a genome length of 3268756bp, was found to be resistant to azithromycin and trimethoprim/sulfamethoxazole but susceptible to tetracycline, ofloxacin, rifampicin, ciprofloxacin and doxycycline. The presence of virulence genes in the strain was identified.
Conclusions: The results obtained will help in understanding drug resistance mechanisms and virulence factors in highly zoonotic B. melitensis and suggest the need for judicious use of antibiotics in livestock health and management practices.
(Copyright © 2018 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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