Sequential bacterial sampling of the midline incision in horses undergoing exploratory laparotomy.

Autor: Isgren CM; Department of Epidemiology and Population Health, Institute of Infection and Global Health, University of Liverpool, Leahurst, UK.; Department of Equine Clinical Science, Institute of Veterinary Science, University of Liverpool, Leahurst, UK., Salem SE; Department of Epidemiology and Population Health, Institute of Infection and Global Health, University of Liverpool, Leahurst, UK.; Department of Surgery, Faculty of Veterinary Medicine, Zagazig University, Zakazik, Egypt., Townsend NB; Three Counties Equine Hospital, Stratford Bridge, Ripple, Tewkesbury, UK., Timofte D; Institute of Veterinary Science, University of Liverpool, Leahurst, UK.; Institute of Infection and Global Health, University of Liverpool, Leahurst, UK., Maddox TW; Department of Musculoskeletal Biology, Institute of Ageing and Chronic Disease, University of Liverpool, Leahurst, UK., Archer DC; Department of Epidemiology and Population Health, Institute of Infection and Global Health, University of Liverpool, Leahurst, UK.; Department of Equine Clinical Science, Institute of Veterinary Science, University of Liverpool, Leahurst, UK.
Jazyk: angličtina
Zdroj: Equine veterinary journal [Equine Vet J] 2019 Jan; Vol. 51 (1), pp. 38-44. Date of Electronic Publication: 2018 May 17.
DOI: 10.1111/evj.12958
Abstrakt: Background: There is limited information about bacterial isolates that are present on the equine midline incision during and following exploratory laparotomy.
Objectives: To investigate the bacterial species cultured from the ventral midline pre-, intra- and post- laparotomy, whether particular bacterial isolates are associated with the development of surgical site infections (SSIs) and to report the antimicrobial resistance phenotypes of these isolates.
Study Design: Prospective cohort study.
Methods: The ventral midline of 31 horses undergoing exploratory laparotomy was sampled for bacterial culture at set time-points pre, intra and post-operatively. Inclusion criteria were that horses must have undergone exploratory laparotomy within 90 min of the initial colic examination upon hospital admission and must not have been placed in a stable prior to surgery. SSI was defined as any purulent or serous discharge from the laparotomy incision of >24 h duration.
Results: Seven horses (22.6%) developed a SSI. None of the variables tested were associated with the altered risk of SSI. The prevalence of a positive bacterial culture from the incision increased progressively over time and a variety of bacteria were isolated. A positive intra-operative culture was not a predictor of SSI; and when a SSI did occur, it was due to a different bacterial isolate. MRSA and ESBL-producers were identified in the post-operative period in one and four different horses respectively, but none of these developed a SSI.
Main Limitations: Sampling was limited to hospitalisation and no culture results were available for horses developing SSI following hospital discharge.
Conclusions: A variety of bacterial species may be isolated from equine laparotomy incisions peri-operatively without development of SSI. SSI does not appear to be solely related to bacterial contamination of the incision peri-operatively and other mechanisms such as bacteraemia merit further investigation.
(© 2018 The Authors. Equine Veterinary Journal published by John Wiley & Sons Ltd on behalf of EVJ Ltd.)
Databáze: MEDLINE
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