| Autor: |
Thomas Pashuck E; Department of Materials Science and Engineering, Imperial College London, London, UK. t.pashuck@gmail.com. |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1758, pp. 177-192. |
| DOI: |
10.1007/978-1-4939-7741-3_14 |
| Abstrakt: |
Peptide self-assembly is an important field in biomaterials in which short peptides are designed to aggregate into nanostructures that often form hydrogels. These peptides are typically made using solid-phase peptide synthesis (SPPS), a technique in which amino acids are added sequentially to a growing chain. This technique has been used to synthesize peptides with more than 100 amino acids. However, self-assembling peptides are designed to aggregate in solution, which often reduces coupling efficiency during synthesis and makes purification more difficult. Here, an outline of solid-phase peptide synthesis is provided, along with steps that can be used to improve the synthetic yield and purification of self-assembling peptides for regenerative medicine applications. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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