Quantitative Proteomic Analysis of Cellular Responses to a Designed Amino Acid Feed in a Monoclonal Antibody Producing Chinese Hamster Ovary Cell Line

Autor:	Torkashvand F; Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran., Mahboudi F; Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran., Vossoughi M; Department of Chemical and Petroleum Engineering, Biochemical and Bioenvironmental Research Center Sharif University of Technology, Tehran, Iran., Fatemi E; Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran., Moosavi Basri SM; Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran., Heydari A; Department of Chemical Engineering, University of Mohaghegh Ardabili, Ardabil, Iran., Vaziri B; Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
Jazyk:	angličtina
Zdroj:	Iranian biomedical journal [Iran Biomed J] 2018 Nov; Vol. 22 (6), pp. 385-93. Date of Electronic Publication: 2018 Apr 21.
Abstrakt:	Background: Chinese hamster ovary (CHO) cell line is considered as the most common cell line in the biopharmaceutical industry because of its capability in performing efficient post-translational modifications and producing the recombinant proteins, which are similar to natural human proteins. The optimization of the upstream process via different feed strategies has a great impact on the target molecule expression and yield. Methods: To determine and understand the molecular events beneath the feed effects on the CHO cell, a label-free quantitative proteomic analysis was applied. The proteome changes followed by the addition of a designed amino acid feed to the monoclonal antibody producing CHO cell line culture medium were investigated. Results: The glutathione synthesis, the negative regulation of the programmed cell death, proteasomal catabolic process, and the endosomal transport pathway were up-regulated in the group fed with a designed amino acid feed compared to the control group. Conclusion: Our findings could be helpful to identify new targets for metabolic engineering.
Databáze:	MEDLINE
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