Circulating miRNAs in acute new-onset atrial fibrillation and their target mRNA network.

Autor: da Silva AMG; Department of Clinical Analysis and Toxicology, Federal University of Rio Grande do Norte, Natal, RN, Brazil., de Araújo JNG; Department of Clinical Analysis and Toxicology, Federal University of Rio Grande do Norte, Natal, RN, Brazil., de Oliveira KM; Department of Clinical Analysis and Toxicology, Federal University of Rio Grande do Norte, Natal, RN, Brazil., Novaes AEM; Department of Integrated Medicine, Hospital Onofre Lopes, Federal University of Rio Grande do Norte, Natal, RN, Brazil., Lopes MB; Department of Clinical Analysis and Toxicology, Federal University of Rio Grande do Norte, Natal, RN, Brazil., de Sousa JCV; Department of Integrated Medicine, Hospital Onofre Lopes, Federal University of Rio Grande do Norte, Natal, RN, Brazil., Filho AAA; Hospital Casa de Saúde São Lucas, Natal, RN, Brazil., Luchessi AD; Department of Clinical Analysis and Toxicology, Federal University of Rio Grande do Norte, Natal, RN, Brazil., de Rezende AA; Department of Clinical Analysis and Toxicology, Federal University of Rio Grande do Norte, Natal, RN, Brazil., Hirata MH; Department of Clinical Analysis and Toxicology, School of Pharmaceutical Sciences, University of Sao Paulo, Sao Paulo, Brazil., Silbiger VN; Department of Clinical Analysis and Toxicology, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
Jazyk: angličtina
Zdroj: Journal of cardiovascular electrophysiology [J Cardiovasc Electrophysiol] 2018 Aug; Vol. 29 (8), pp. 1159-1166. Date of Electronic Publication: 2018 May 09.
DOI: 10.1111/jce.13612
Abstrakt: Background: MicroRNAs (miRNAs) are involved in the pathogenesis of atrial fibrillation (AF), acting on development and progression. Our pilot study investigated the expression of six miRNAs and their miRNA-mRNA interactions in patients with acute new-onset AF, well-controlled AF, and normal sinus rhythm (controls).
Methods and Results: Plasma of acute new-onset AF patients (n = 5) was collected in the emergency room when patients presented with irregular and fast-atrial fibrillation rhythm. Samples from well-controlled AF (n = 16) and control (n =  15) patients were collected during medical appointments following an ECG. Expression of miR-21, miR-133a, miR-133b, miR-150, miR-328, and miR-499 was analyzed by real-time PCR. Ingenuity Pathway Analysis and the TargetScan database identified the top 30 mRNA targets of these miRNA, seeking the miRNA-mRNA interactions in cardiovascular process. Increased expression of miR-133b (1.4-fold), miR-328 (2.0-fold), and miR-499 (2.3-fold) was observed in patients with acute new-onset AF, compared with well-controlled AF and control patients. Decreased expression of miR-21 was seen in patients with well-controlled AF compared to those with acute new-onset AF and controls (0.6-fold). The miRNA-mRNA interaction demonstrated that SMAD7 and FASLG genes were the targets of miR-21, miR-133b, and miR-499 and were directly related to AF, being involved in apoptosis and fibrosis.
Conclusion: The miRNAs had different expression profiles dependent on the AF condition, with higher expression in the acute new-onset AF than well-controlled AF. Clinically, this may contribute to an effective assessment for patients, leading to early detection of AF and monitoring to reduce the risk of other serious cardiovascular events.
(© 2018 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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