Cassia grandis fruit extract reduces the blood glucose level in alloxan-induced diabetic rats.
| Autor: | Prada AL; Pharmacy Departament, University of Oriente, Santiago de Cuba, Cuba; Drugs Research Laboratory, Federal University of Amapá, Rodovia JK, km 2, Maro zero Ecuador, Macapá, Amapá, Brazil., Amado JRR; Pharmacy Departament, University of Oriente, Santiago de Cuba, Cuba; Laboratory of Innovation and Development in Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of Amazonas, Ave. Rodrigo Otavio Ramos 6200, Coroado, CEP 69077-000, Manaus, AM, Brazil. Electronic address: jiribilla2009@gmail.com., Keita H; University of La sierra Sur, Division of Postgraduate Research, Institute of Public Health, Calle Guillermo Rojas Mijangos S/N, Ciudad Universitaria, Miahuatlán de Porfirio Díaz, Oaxaca, Mexico., Zapata EP; Toxicology and Biomedicine Center, Medical University of Santiago de Cuba, Santiago de Cuba, Cuba., Carvalho H; Drugs Research Laboratory, Federal University of Amapá, Rodovia JK, km 2, Maro zero Ecuador, Macapá, Amapá, Brazil., Lima ES; Laboratory of Biological Activity, Faculty of Pharmaceutical Sciences, University of Amazonas, Ave. Rodrigo Otavio Ramos 6200, Coroado, CEP 69077-000, Manaus, AM, Brazil., de Sousa TP; Laboratory of Innovation and Development in Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of Amazonas, Ave. Rodrigo Otavio Ramos 6200, Coroado, CEP 69077-000, Manaus, AM, Brazil., Carvalho JCT; Drugs Research Laboratory, Federal University of Amapá, Rodovia JK, km 2, Maro zero Ecuador, Macapá, Amapá, Brazil. |
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| Jazyk: | angličtina |
| Zdroj: | Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2018 Jul; Vol. 103, pp. 421-428. Date of Electronic Publication: 2018 Apr 24. |
| DOI: | 10.1016/j.biopha.2018.04.059 |
| Abstrakt: | Introduction: Cassia grandis Lf fruits are ethnobotanically used for digestive disorders, anemia, and for reducing blood glucose. However, there are no studies about the antidiabetic activity nor the oral toxicity of the plant fruit-extracts. This paper aims to evaluate the hypoglycemic effect of C. grandis fruits extract in vivo, and assess the acute oral toxicity, and sub-acute oral toxicity. The antioxidant activity and the α-glycosidase inhibitor effect were also evaluated. Methods: The extract was obtained by maceration of the fruit pulp with 70% hydroalcoholic solution (1:2, m:v). The extractive solution was concentrated in a vacuum rotary evaporator, up to a drug: solvent ratio of 2:1 (g/ml). Soluble solids, relative density, refractive index, pH, total phenolics, and flavonoids were determined. A preliminary phytochemical screening was made, followed by the quantitation of volatiles by GC/MS. The acute and sub-acute oral toxicity was evaluated in Sprague Dawley rats, by using biochemical and hematological parameters. The radical scavenging activity (DPPH, ABTS) and α-glycosidase inhibitory effect were tested. The hypoglycemic effect was assessed in alloxan-induced diabetic rats. Results: The extract of C. grandis contains alkaloids, coumarins, flavonoids, free amino acids, amines, phenols, tannins, reduced sugars, resins, saponins, steroids, and triterpenes, plus 38 volatile compounds, being linalool the most abundant (1,66%). The extract exhibited an LD Conclusion: C. grandis extract is an excellent hypoglycemic and non-toxic plant product. The hypoglycemic mechanism could be associated with the antioxidant effect and with the α-glycosidase inhibition. Up to the best of our knowledge, this is the first report on the hypoglycemic effect in vivo of C. grandis fruits extract. (Copyright © 2018 Elsevier Masson SAS. All rights reserved.) |
| Databáze: | MEDLINE |
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