Gene-based molecular analysis of COX1 in Echinococcus granulosus cysts isolated from naturally infected livestock in Riyadh, Saudi Arabia.

Autor: Metwally DM; Zoology Department, Faculty of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.; Parasitology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt., Qassim LE; Zoology Department, Faculty of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia., Al-Turaiki IM; Information Technology Department, College of Computer and Information Science, King Saud University, Riyadh, Kingdom of Saudi Arabia., Almeer RS; Zoology Department, Faculty of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia., El-Khadragy MF; Zoology Department, Faculty of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.; Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo, Egypt.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2018 Apr 18; Vol. 13 (4), pp. e0195016. Date of Electronic Publication: 2018 Apr 18 (Print Publication: 2018).
DOI: 10.1371/journal.pone.0195016
Abstrakt: The diversity and importance of Echinococcus species in domesticated animals in Saudi Arabia are poorly understood. In this study, 108 singular (hydatid) cysts were collected from goats (n = 25), sheep (n = 56) and camels (n = 27). DNA was extracted from the protoscoleces of individual fertile cysts and used for polymerase chain reaction (PCR) amplification of mitochondrial subunit 1 of the cytochrome c oxidase 1 (cox1) gene. Amplicon sequencing results revealed the presence of Echinococcus granulosus sensustricto (s.s.) (genotypes G1-G3) in 16 of the17 sheep cysts and 2 of the 27 camel cysts.of these samples, 18 (2 camel and 16 sheep) were divided into genotypes G1, G2, and G3.
Databáze: MEDLINE
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