Evaluation effects of allopurinol and FSH on reduction of ischemia-reperfusion injury and on preservation of follicle after heterotopic auto-transplantation of ovarian tissue in mouse.

Autor: Abedi R; Department of Embryology at Reproductive Biomedicine Research Center Royan Institute for Reproductive Biomedicine, ACECR 19395-4644 Tehran Iran.; Department of Biology, Faculty of Biological Science Shahid Beheshti University Tehran Iran., Eimani H; Department of Embryology at Reproductive Biomedicine Research Center Royan Institute for Reproductive Biomedicine, ACECR 19395-4644 Tehran Iran.; Department of Anatomy, Faculty of Medicine Baqiyatallah University of Medical Science TehranIran., Pashaee Rad S; Department of Biology, Faculty of Biological Science Shahid Beheshti University Tehran Iran., Eftekhari Yazdi P; Department of Embryology at Reproductive Biomedicine Research Center Royan Institute for Reproductive Biomedicine, ACECR 19395-4644 Tehran Iran., Shahverdi AH; Department of Embryology at Reproductive Biomedicine Research Center Royan Institute for Reproductive Biomedicine, ACECR 19395-4644 Tehran Iran., Mokhber Maleki E; Department of Embryology at Reproductive Biomedicine Research Center Royan Institute for Reproductive Biomedicine, ACECR 19395-4644 Tehran Iran.; Department of Biology, Faculty of Biological Science Shahid Beheshti University Tehran Iran.
Jazyk: angličtina
Zdroj: Reproductive medicine and biology [Reprod Med Biol] 2013 Jul 24; Vol. 13 (1), pp. 29-35. Date of Electronic Publication: 2013 Jul 24 (Print Publication: 2014).
DOI: 10.1007/s12522-013-0160-2
Abstrakt: Purpose: Allopurinol and FSH injection are applied to reduce ischemia-reperfusion injury and to increase survival rate for ovarian follicles after ovarian heterotopic autotransplantation in mice.
Methods: Ovarian tissues from 6-week-old mice were grafted into back muscle then collected after 3 weeks. A total of five groups were included in this experiment as follows: control group ( n = 5), sham-operated group ( n = 5), allopurinol treatment group (AP) ( n = 5), follicle stimulating hormone (FSH) treatment group ( n = 5), as well as, allopurinol and FSH treatment group (APF) ( n = 5). We investigated survival, number and development of follicles, vaginal cytology along with plasma malondialdehyde (MDA) concentration in grafted ovary.
Results: Total follicles count significantly increased in APF group compared with other treatment groups ( p < 0.05). MDA concentration significantly decreased in AP group and APF treatment group compared with sham-operated group. In AP group, vaginal smears showed presence of cornified epithelial cells three-five day after surgery.
Conclusions: We demonstrated that allopurinol, as a XO inhibitor, plays an important role in order to decrease ischemia injury and to increase survival rate for follicles. Also, FSH, as a folliculogenesis and angiogenesis factor, increases development of follicles. It seems that allopurinol can cause re-establishing hypothalamus-pituitary axis and finally can restore estrous cycle earlier than for the sham operated group, so it explains the increasing survival rate for follicles.
Databáze: MEDLINE
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