DNA damaging and apoptotic potentials of Bisphenol A and Bisphenol S in human bronchial epithelial cells.

Autor: George VC; Department of Plant, Food, and Environmental Sciences, Faculty of Agriculture, Dalhousie University, Truro, Nova Scotia, Canada., Rupasinghe HPV; Department of Plant, Food, and Environmental Sciences, Faculty of Agriculture, Dalhousie University, Truro, Nova Scotia, Canada; Department of Pathology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada. Electronic address: vrupasinghe@dal.ca.
Jazyk: angličtina
Zdroj: Environmental toxicology and pharmacology [Environ Toxicol Pharmacol] 2018 Jun; Vol. 60, pp. 52-57. Date of Electronic Publication: 2018 Apr 11.
DOI: 10.1016/j.etap.2018.04.009
Abstrakt: DNA damage caused by environmental agents often lead to many chronic diseases, including cancer. The present study aimed to understand the relative toxicity possessed by Bisphenol A (BPA) and Bisphenol S (BPS) on human bronchial epithelial cells (BEAS-2B). The cells were exposed to either BPA or BPS and evaluated for its cytotoxicity, reactive oxygen species (ROS), DNA fragmentation, phosphorylated histone protein (γ-H2AX) and DNA tail damage levels. Further, we also studied DNA damage response (DDR) and caspase-3 mechanisms, to evaluate its mechanism of cell death processes. Exposure with 200 μM of BPA, significantly (p < 0.05) induces caspase-3-mediated cell death by inducing cytotoxicity, ROS, and DNA fragmentation. Higher levels of γ-H2AX and DNA tail damage indicated BPA's DNA damaging potential through an ATM/ATR/Chk1/p53-dependent pathway in BEAS-2B cells. Overall, in vitro data exhibited moderate toxicity for BPS in comparison with BPA suggesting the need for a thorough clinical investigation over its safety profile.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE